https://scholars.lib.ntu.edu.tw/handle/123456789/425910
標題: | Diffusion tensor tractography reveals muscle reconnection during axolotl limb regeneration | 作者: | CHENG-HAN WU YU-JEN CHEN Wang, Mu Hui Chiou, Ling Ling WEN-YIH TSENG Lee, Hsuan-Shu |
公開日期: | 1-三月-2017 | 卷: | 12 | 期: | 3 | 來源出版物: | PLoS ONE | 摘要: | ©2017 Wu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Axolotls have amazing ability to regenerate their lost limbs. Our previous works showed that after amputation the remnant muscle ends remained at their original location whilst sending satellite cells into the regenerating parts to develop into early muscle fibers in the late differentiation stage. The parental and the newly formed muscle fibers were not connected until very late stage. The present study used non-invasive diffusion tensor imaging (DTI) to monitor weekly axolotl upper arm muscles after amputation of their upper arms. DTI tractography showed that the regenerating muscle fibers became visible at 9-wpa (weeks post amputation), but a gap was observed between the regenerating and parental muscles. The gap was filled at 10-wpa, indicating reconnection of the fibers of both muscles. This was confirmed by histology. The DTI results indicate that 23% of the muscle fibers were reconnected at 10-wpa. In conclusion, DTI can be used to visualize axolotls' skeletal muscles and the results of muscle reconnection were in accordance with our previous findings. This non-invasive technique will allow researchers to identify the timeframe in which muscle fiber reconnection takes place and thus enable the study of the mechanisms underlying this reconnection. |
URI: | https://www2.scopus.com/inward/record.uri?eid=2-s2.0-85014495533&doi=10.1371%2fjournal.pone.0173425&partnerID=40&md5=74c0a6482c3ee2c13287005a0778e6be https://scholars.lib.ntu.edu.tw/handle/123456789/425910 |
ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0173425 https://api.elsevier.com/content/abstract/scopus_id/85014495533 |
顯示於: | 醫療器材與醫學影像研究所 |
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