Effects of antrosterol from Antrodia camphorata submerged whole broth on lipid homeostasis, antioxidation, alcohol clearance, anti-inflammation in livers of chronic-alcohol fed mice
Journal
Journal of Ethnopharmacology
Journal Volume
202
Pages
200-207
Date Issued
2017
Author(s)
Abstract
Ethnopharmacological relevance Antrodia camphorata is a functional fungus in Taiwan and owns several pharmacological functions. Antrosterol, a bioactive constitute of sterols in edible Antrodia camphorata submerged whole broth, can protect liver from CCl4 damage via enhancing antioxidant and anti-inflammatory capacities. Aim of the study The aim of this study was to investigate the hepatoprotection of antrosterol (named as EK100) against alcohol consumption. Materials and methods A Lieber-DeCarli regular EtOH diet (EtOH liquid diet, 5% (v/v) alcohol) was applied to induce alcoholic liver damage. Mice were randomly divided into 5 groups: (1) Control: control liquid diet; (2) EtOH: EtOH liquid diet; (3) EK100_1X: EtOH liquid diet and 1?mg EK100 (Antrosterol)/Kg body weight (bw); (4) EK100_5X: EtOH liquid diet and 5?mg EK100/Kg bw; (5) EK100_10X: EtOH liquid diet and 10?mg EK100/Kg bw. At the end of experiment, the livers were collected for histo-pathological analyses, RNA and protein extraction, and enzymatic activities. Results Antrosterol reduced serum/liver lipids of alcohol-diet fed mice which highly related to upregulated fatty acid β-oxidation and downregulated lipogenesis, and increased fecal lipid/bile-acid outputs. Antrosterol enhanced hepatic antioxidant capabilities in alcohol-diet fed mice while it also lowered serum alcohol level, as well as increased alcohol dehydrogenase (ADH) and catalase (CAT) activities and decreased CYP2E1 protein expression in livers of alcohol-diet fed mice. Besides, antrosterol lowered hepatic inflammation and fibrosis related gene expressions, as well as serum AST/ALT values and TNF-α/IL-1β contents in alcohol-diet fed mice. Conclusion Based on the results, hepatoprotection of antrosterol is mostly attributed to its regulations of lipid homeostasis, antioxidant capability, alcohol metabolism, and anti-inflammation. ? 2017 Elsevier Ireland Ltd
Subjects
Alcohol metabolism; Alcoholic liver disease; Antioxidant/anti-inflammation; Antrosterol; Lipid homeostasis
SDGs
Other Subjects
alanine aminotransferase; alcohol; alcohol dehydrogenase; alpha smooth muscle actin; antrosterol; aspartate aminotransferase; bile acid; carnitine palmitoyltransferase I; catalase; cyclooxygenase 2; cytochrome P450 2E1; feces lipid; glutathione; immunoglobulin enhancer binding protein; interleukin 1beta; liver protective agent; myeloid differentiation factor 88; peroxisome proliferator activated receptor alpha; retinoid X receptor alpha; sterol regulatory element binding protein 1c; thiobarbituric acid reactive substance; toll like receptor 4; tumor necrosis factor; unclassified drug; uncoupling protein 2; alcohol; antioxidant; antrosterol; central depressant agent; cytokine; fatty acid; nonsteroid antiinflammatory agent; sterol; alanine aminotransferase blood level; alcohol blood level; alcohol consumption; alcohol liver cirrhosis; alcohol metabolism; animal experiment; animal model; animal tissue; antiinflammatory activity; antioxidant activity; Antrodia camphorata; Article; aspartate aminotransferase blood level; controlled study; down regulation; drug efficacy; enzyme activity; enzyme regulation; fatty acid oxidation; gene expression; histopathology; lipid blood level; lipid diet; lipid homeostasis; lipid liver level; lipogenesis; liver protection; liver tissue; male; mouse; nonhuman; oxidative stress; protein content; protein expression; upregulation; animal; Antrodia; blood; C57BL mouse; chemistry; diet; drug effects; growth; Hepatitis, Alcoholic; lipid metabolism; liver; liver function test; metabolism; pathology; Animals; Anti-Inflammatory Agents, Non-Steroidal; Antioxidants; Antrodia; Central Nervous System Depressants; Cytokines; Diet; Ethanol; Fatty Acids; Growth; Hepatitis, Alcoholic; Lipid Metabolism; Liver; Liver Function Tests; Male; Mice; Mice, Inbred C57BL; Sterols
Type
journal article