https://scholars.lib.ntu.edu.tw/handle/123456789/426461
標題: | Clathrin-coat disassembly illuminates the mechanisms of Hsp70 force generation | 作者: | Sousa, Rui HSIEN-SHUN LIAO Cuéllar, Jorge Jin, Suping Valpuesta, José M Jin, Albert J Lafer, Eileen M |
公開日期: | 2016 | 出版社: | NATURE PUBLISHING GROUP | 卷: | 23 | 期: | 9 | 起(迄)頁: | 821 | 來源出版物: | Nature structural & molecular biology | 摘要: | Hsp70s use ATP hydrolysis to disrupt protein-protein associations and to move macromolecules. One example is the Hsc70- mediated disassembly of the clathrin coats that form on vesicles during endocytosis. Here, we exploited the exceptional features of these coats to test three models-Brownian ratchet, power-stroke and entropic pulling-proposed to explain how Hsp70s transform their substrates. Our data rule out the ratchet and power-stroke models and instead support a collision-pressure mechanism whereby collisions between clathrin-coat walls and Hsc70s drive coats apart. Collision pressure is the complement to the pulling force described in the entropic pulling model. We also found that self-association augments collision pressure, thereby allowing disassembly of clathrin lattices that have been predicted to be resistant to disassembly. These results illuminate how Hsp70s generate the forces that transform their substrates. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/426461 | ISSN: | 1545-9993 | DOI: | https://api.elsevier.com/content/abstract/scopus_id/84980325655 10.1038/nsmb.3272 |
顯示於: | 機械工程學系 |
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