https://scholars.lib.ntu.edu.tw/handle/123456789/426461
Title: | Clathrin-coat disassembly illuminates the mechanisms of Hsp70 force generation | Authors: | Sousa, Rui HSIEN-SHUN LIAO Cuéllar, Jorge Jin, Suping Valpuesta, José M Jin, Albert J Lafer, Eileen M |
Issue Date: | 2016 | Publisher: | NATURE PUBLISHING GROUP | Journal Volume: | 23 | Journal Issue: | 9 | Start page/Pages: | 821 | Source: | Nature structural & molecular biology | Abstract: | Hsp70s use ATP hydrolysis to disrupt protein-protein associations and to move macromolecules. One example is the Hsc70- mediated disassembly of the clathrin coats that form on vesicles during endocytosis. Here, we exploited the exceptional features of these coats to test three models-Brownian ratchet, power-stroke and entropic pulling-proposed to explain how Hsp70s transform their substrates. Our data rule out the ratchet and power-stroke models and instead support a collision-pressure mechanism whereby collisions between clathrin-coat walls and Hsc70s drive coats apart. Collision pressure is the complement to the pulling force described in the entropic pulling model. We also found that self-association augments collision pressure, thereby allowing disassembly of clathrin lattices that have been predicted to be resistant to disassembly. These results illuminate how Hsp70s generate the forces that transform their substrates. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/426461 | ISSN: | 1545-9993 | DOI: | https://api.elsevier.com/content/abstract/scopus_id/84980325655 10.1038/nsmb.3272 |
Appears in Collections: | 機械工程學系 |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.