https://scholars.lib.ntu.edu.tw/handle/123456789/431081
標題: | Functional domains of delta antigens and viral RNA required for RNA packaging of hepatitis delta virus | 作者: | MING-FU CHANG Chen C.-H. Lin S.-L. Chen C.-J. SHIN CHANG |
公開日期: | 1995 | 卷: | 69 | 期: | 4 | 起(迄)頁: | 2508-2514 | 來源出版物: | Journal of Virology | 摘要: | The functions of delta antigens (HDAgs) in the morphogenesis of hepatitis delta virus (HDV) have been studied previously. The C terminus of large HDAg has been shown to complex with the small surface antigen (HBsAg) of helper hepatitis B virus, whereas the assembly of small HDAg requires interaction with the N terminus of large HDAg (M.-F. Chang, C.-J. Chen, and S.C. Chang, J. Virol. 68:646-653, 1994). To further examine the molecular mechanisms by which HDAgs are involved in the assembly of HDV RNA, we have cotransfected Huh-7 cells with plasmids representing a longer than unit-length HDV and the small HBsAg cDNAs. We found that HDAg mRNA could be generated from an endogenous promoter within the HDV cDNA that was translated into large HDAg. Large HDAg is capable of complexing with monomeric HDV genomic RNA to form ribonucleoprotein particles (RNPs) and is capable of forming enveloped HDV- like particles in the presence of small HBsAg without undergoing HDV replication. In addition, the middle region from amino acid residues 89 to 145 of large HDAg is required for assembly of the RNPs but is dispensable for assembly of the enveloped particles. RNA assembly is also demonstrated with small HDAg when it is cotransfected with a packaging-defective large HDAg mutant and small HBsAg. Leu-115 within the putative helix-loop-helix structure of the small HDAg is important for the replication of HDV but is not essential for RNA assembly, suggesting that conformational requirements of small HDAg for replication and assembly of viral RNA may be different. Further studies indicate that a 312-nucleotide linear HDV RNA from one end of the HDV rod structure is sufficient to form RNP complexes competent for assembly of virus-like particles with large HDAg and small HBsAg. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028940476&partnerID=40&md5=c3648c4e6b98c6f65a7dc569a30a5ca4 https://scholars.lib.ntu.edu.tw/handle/123456789/431081 |
SDG/關鍵字: | complementary DNA; hepatitis B surface antigen; hepatitis delta antigen; messenger RNA; ribonucleoprotein; virus RNA; amino acid sequence; article; Hepatitis delta virus; hepatoma cell; human; human cell; nonhuman; nucleotide sequence; plasmid; priority journal; promoter region; protein assembly; viral genetics; virus assembly; virus replication |
顯示於: | 生物化學暨分子生物學科研究所 |
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