The regulatory effects of fish oil and chitosan on hepatic lipogenic signals in high-fat diet-induced obese rats
Journal
Journal of Food and Drug Analysis
Journal Volume
25
Journal Issue
4
Pages
919-930
Date Issued
2017
Author(s)
Abstract
The present study investigated the regulatory effects of fish oil and chitosan on the signals of hepatic lipid metabolism and the postulated mechanism in high-fat diet-induced obese rats. Diet supplementation of chitosan and fish oil efficiently suppressed the increased weights in body and livers of high-fat diet-fed rats. Supplementation of chitosan and fish oil significantly decreased the activities of hepatic lipid biosynthesis-related enzymes and efficiently regulated plasma lipoprotein homeostasis. Both chitosan and fish oil significantly ameliorated the alterations in the protein expressions of hepatic lipogenic transcription factors (LXRα and PPARα), and could also significantly regulate the downstream hepatic lipogenic genes (FAS, HMGCR, CYP7A1, FATP, FABP, AOX, and ABCA) expressions in high-fat diet-fed rats. These results suggest that both fish oil and chitosan exerts downregulative effects on hepatic lipid metabolism in high-fat diet-induced obese rats via the LXRα inhibition and PPARα activation, which further affect the expressions of hepatic lipogenesis-associated genes. ? 2017
Subjects
chitosan; fish oil; gene expressions; high-fat diet; lipogenesis
SDGs
Other Subjects
chitosan; cholesterol 7alpha monooxygenase; fatty acid binding protein; fatty acid synthase; fatty acid transporter; fish oil; high density lipoprotein cholesterol; lipid; lipogenic transcription factor alpha; lipoprotein; liver X receptor alpha; peroxisome proliferator activated receptor alpha; transcription factor; unclassified drug; very low density lipoprotein cholesterol; chitosan; cholesterol 7alpha monooxygenase; CYP7A1 protein, rat; fatty acid binding protein; fish oil; peroxisome proliferator activated receptor alpha; Slc27a1 protein, rat; abca gene; animal experiment; animal model; animal tissue; aox gene; Article; controlled study; diet induced obesity; diet supplementation; enzyme activation; fatty acid synthesis; gene; gene expression; hmgcr gene; lipid composition; lipid diet; lipid homeostasis; lipid liver level; lipid metabolism; lipogenesis; lipoprotein blood level; male; nonhuman; protein expression; rat; animal; drug effects; genetics; human; lipid diet; lipogenesis; liver; metabolism; obesity; Sprague Dawley rat; Animals; Chitosan; Cholesterol 7-alpha-Hydroxylase; Diet, High-Fat; Fatty Acid-Binding Proteins; Fish Oils; Humans; Lipid Metabolism; Lipogenesis; Liver; Male; Obesity; PPAR alpha; Rats; Rats, Sprague-Dawley
Publisher
Elsevier Taiwan LLC
Type
journal article