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  4. The osteoblastogenesis potential of adipose mesenchymal stem cells in myeloma patients who had received intensive therapy
 
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The osteoblastogenesis potential of adipose mesenchymal stem cells in myeloma patients who had received intensive therapy

Journal
PLoS ONE
Journal Volume
9
Journal Issue
4
Date Issued
2014
Author(s)
Lin H.-H.
Hwang S.-M.
SHANG-JU WU  
Hsu L.-F.
YI-HUA LIAO  
YI-SHUAN SHEEN  
Chuang W.-H.
SHANG-YI HUANG  
DOI
10.1371/journal.pone.0094395
URI
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84899512979&doi=10.1371%2fjournal.pone.0094395&partnerID=40&md5=ddc438b93a714731a3d62435536842e3
https://scholars.lib.ntu.edu.tw/handle/123456789/434705
Abstract
Multiple myeloma (MM) is characterized by advanced osteolytic lesions resulting from the activation of osteoclasts (OCs) and inhibition of osteoblasts (OBs). OBs are derived from mesenchymal stem cells (MSCs) from the bone marrow (BM), however the pool and function of BMMSCs in MM patients (MM-BMMSCs) are reduced by myeloma cells (MCs) and cytokines secreted from MCs and related anti-MM treatment. Such reduction in MM-BMMSCs currently cannot be restored by any means. Recently, genetic aberrations of MM-BMMSCs have been noted, which further impaired their differentiation toward OBs. We hypothesize that the MSCs derived from adipose tissue (ADMSCs) can be used as alternative MSC sources to enhance the pool and function of OBs. Therefore, the purpose of this study was to compare the osteogenesis ability of paired ADMSCs and BMMSCs in MM patients who had completed intensive therapy. Fifteen MM patients who had received bortezomib-based induction and autologous transplantation were enrolled. At the third month after the transplant, the paired ADMSCs and BMMSCs were obtained and cultured. Compared with the BMMSCs, the ADMSCs exhibited a significantly higher expansion capacity (100% vs 13%, respectively; P = .001) and shorter doubling time (28 hours vs 115 hours, respectively; P = .019). After inducing osteogenic differentiation, although the ALP activity did not differ between the ADMSCs and BMMSCs (0.78 U/μg vs 0.74±0.14 U/μg, respectively; P = .834), the ADMSCs still exhibited higher calcium mineralization, which was determined using Alizarin red S (1029 nmole vs 341 nmole, respectively; P = .001) and von Kossa staining (2.6 E+05 μm2 vs 5 E+04 μm2, respectively; P = .042), than the BMMSCs did. Our results suggested that ADMSCs are a feasible MSC source for enhancing the pool and function of OBs in MM patients who have received intensive therapy. ? 2014 Lin et al.
SDGs

[SDGs]SDG3

Other Subjects
5' nucleotidase; alizarin red s; alkaline phosphatase; beta galactosidase; bortezomib; calcium; CD14 antigen; CD19 antigen; CD34 antigen; CD45 antigen; cyclophosphamide; dexamethasone; doxorubicin; endoglin; Hermes antigen; HLA DR antigen; melphalan; syndecan 1; thalidomide; Thy 1 antigen; antineoplastic agent; antineoplastic agent; boronic acid derivative; bortezomib; cycloheximide; dexamethasone; pyrazine derivative; thalidomide; adipose derived stem cell; adult; aged; article; autologous stem cell transplantation; biomineralization; bone development; cancer chemotherapy; cell differentiation; cell expansion; clinical article; controlled study; female; hematopoietic stem cell; human; human cell; male; mesenchymal stem cell; middle aged; multiple cycle treatment; multiple myeloma; adipose tissue; autotransplantation; bone marrow cell; cell culture; cell proliferation; cytology; hematopoietic stem cell transplantation; immunology; mesenchymal stroma cell; multiple myeloma; osteoblast; pathology; remission; transplantation; Adipose Tissue; Adult; Aged; Antineoplastic Agents; Antineoplastic Combined Chemotherapy Protocols; Bone Marrow Cells; Boronic Acids; Cell Differentiation; Cell Proliferation; Cells, Cultured; Cycloheximide; Dexamethasone; Female; Hematopoietic Stem Cell Transplantation; Humans; Male; Mesenchymal Stromal Cells; Middle Aged; Multiple Myeloma; Osteoblasts; Osteogenesis; Pyrazines; Remission Induction; Thalidomide; Transplantation, Autologous
Publisher
Public Library of Science
Type
journal article

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