https://scholars.lib.ntu.edu.tw/handle/123456789/434752
標題: | Defective β1-integrins expression in arsenical keratosis and arsenic-treated cultured human keratinocytes | 作者: | Lee C.-H. JAU-SHIUH CHEN Sun Y.-L. Liao W.-T. Zheng Y.-W. Chai C.-Z. Chen G.-S. Yu H.-S. |
公開日期: | 2006 | 卷: | 33 | 期: | 2 | 起(迄)頁: | 129-138 | 來源出版物: | Journal of Cutaneous Pathology | 摘要: | Background: β1-integrins, which localize to the basolateral surface of basal keratinocytes, are important in the differentiation control and proliferation of the epidermis. Many cutaneous diseases with perturbed differentiation, including arsenical keratosis, show altered patterns of integrin distribution and expression. Arsenic may induce arsenical keratosis through the differentiation and apoptosis aberration by integrins. The purpose of this study is to investigate the role of integrin and arsenic in the pathogenesis of arsenical keratosis. Methods: Twenty-five specimens obtained from 25 patients with arsenical keratosis disease were studied. Immunohistochemistry staining to β1, α2β 1, or α3β1 integrins was performed in arsenical keratosis and clinically normal perilesional skin. Western blotting was used to assess the expression of integrin β1 and focal adhesion kinase (FAK) in arsenic-treated cultured keratinocytes. Results: A decreased expression of β1, α2β 1, or α3β1 integrins was demonstrated in arsenical keratosis and clinical normal perilesional skin in a large proportion of arsenical keratosis cases studied. The expressions of integrin β1 and FAK were both decreased in arsenic-treated keratinocytes. Conclusions: Our results suggest that arsenic induces abnormal differentiation in arsenical keratosis via the effects of integrin expression in keratinocytes. ? Blackwell Munksgaard 2006. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-33644910477&doi=10.1111%2fj.0303-6987.2006.00361.x&partnerID=40&md5=e4634efbe02124eda497f3182b06978e https://scholars.lib.ntu.edu.tw/handle/123456789/434752 |
ISSN: | 0303-6987 | DOI: | 10.1111/j.0303-6987.2006.00361.x | SDG/關鍵字: | arsenic; beta1 integrin; focal adhesion kinase; very late activation antigen 2; arsenic; beta1 integrin; adult; aged; arsenic poisoning; article; clinical article; female; human; human cell; human tissue; immunohistochemistry; keratinocyte; keratosis; male; pathogenesis; protein expression; skin defect; Western blotting; arsenic poisoning; biosynthesis; cell culture; drug effect; metabolism; middle aged; pathology; reverse transcription polymerase chain reaction; Aged; Aged, 80 and over; Antigens, CD29; Arsenic; Arsenic Poisoning; Blotting, Western; Cells, Cultured; Female; Focal Adhesion Protein-Tyrosine Kinases; Humans; Immunohistochemistry; Keratinocytes; Keratosis; Male; Middle Aged; Reverse Transcriptase Polymerase Chain Reaction |
顯示於: | 醫學系 |
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