Evaluation of the In Vivo Therapeutic Effects of Radix Paeoniae Rubra Ethanol Extract with the Hypoglycemic Activities Measured from Multiple Cell-Based Assays
Journal
Evidence-based Complementary and Alternative Medicine
Journal Volume
2016
Pages
3262790
Date Issued
2016
Author(s)
Yuan, W.; Lin, Y.-L.; Liu, R.-S.; Juan, Y.-C.; Sun, W.-H.; Tsay, H.J.; Huang, H.-C.; Lee, Y.-C.; Liu, H.-K.
Abstract
Background. Radix Paeoniae Rubra (Chi Shao) contains several phytochemicals with hypoglycemic actions. Current research aims to explore potential insulinotropic effects and long-term therapeutic efficacy of such herb against type 2 diabetes. Methods. Composition analysis for the ethanol extract (PRExt) was executed by high performance liquid chromatography. Polyphenol-enriched fraction was characterized by high pressure size exclusion chromatography. Multiple cell platforms were employed to evaluate hypoglycemic bioactivities. In animal experiments, blood glucose, the homeostasis model assessment (HOMA)-index assessment, glucose tolerance test, and in vivo glucose uptake were all measured. Additional effects of PRExt on obesity and hepatic steatosis were evaluated by serum and histological analysis. Results. PRExt provides multiple hypoglycemic effects including the enhancement of glucose-mediated insulin secretion. Pentagalloylglucose and polyphenol-enriched fraction are two insulinotropic constituents. Moreover, PRExt intraperitoneal injection causes acute hypoglycemic effects on fasted db/db mice. Oral administration of PRExt (200 mg/kg b.w.) gradually reduces blood glucose in db/db mice to the level similar to that in C57J/B6 mice after 30 days. The improvement of glucose intolerance, HOMA-index, and in vivo glucose uptake is evident in addition to the weight loss effect and attenuation of hepatic steatosis. Conclusion. PRExt is an effective antidiabetic herbal extract with multiple hypoglycemic bioactivities. ? 2016 Chia-Chuan Chang et al.
SDGs
Other Subjects
alanine aminotransferase; cholesterol; exendin 4; glucose; glucose pentagallate; insulin; Paeonia radix; polyphenol; triacylglycerol; animal cell; animal experiment; animal model; antidiabetic activity; Article; biological activity; blood analysis; cell assay; clinical evaluation; controlled study; drug efficacy; drug screening; fatty liver; glucose blood level; glucose intolerance; glucose tolerance test; glucose transport; high performance liquid chromatography; histopathology; homeostasis; homeostasis model assessment index; hyperglycemia; in vivo study; insulin like activity; insulin release; male; metabolic parameters; mouse; non insulin dependent diabetes mellitus; nonhuman; obesity; pancreas islet beta cell; size exclusion chromatography; therapy effect; weight reduction
Type
journal article