Segmentation of nucleus and cytoplasm of a single cell in three-dimensional tomogram using optical coherence tomography
Journal
Journal of Biomedical Optics
Journal Volume
22
Journal Issue
3
Date Issued
2017
Author(s)
Chang C.-K.
Tsai C.-C.
Hsu W.-Y.
Lin M.-Y.
Tjiu J.-W.
Huang S.-L.
Abstract
A random rayburst sampling (RRBS) framework was developed to detect the nucleus and cell membrane boundaries in three-dimensional (3-D) space. Raw images were acquired through a full-field optical coherence tomography system with submicron resolution - i.e., 0.8 μm in lateral and 0.9 μm in axial directions. The near-isometric resolution enables 3-D segmentation of a nucleus and cell membrane for determining the volumetric nuclear-to-cytoplasmic (N/C) ratio of a single cell. The RRBS framework was insensitive to the selection of seeds and image pixel noise. The robustness of the RRBS framework was verified through the convergence of the N/C ratio searching algorithm. The relative standard deviation of the N/C ratio between different randomly selected seed sets was only 2%. This technique is useful for various in vitro assays on single-cell analyses.
SDGs
Publisher
SPIE
Type
journal article