The effects of marine farm-scale sequentially integrated multi-trophic aquaculture systems on microbial community composition, prevalence of sulfonamide-resistant bacteria and sulfonamide resistance gene sul1
Journal
Science of the Total Environment
Journal Volume
643
Pages
681-691
Date Issued
2018
Author(s)
Abstract
Aquaculture, one of the most important food production practices worldwide, faces serious challenges of mitigating the detrimental impacts of intensive farming on the environment and increased prevalence of antibiotic resistance. To develop an environment-friendly aquaculture system, a land-based and farm-scale sequentially integrated multi-trophic aquaculture (IMTA) system was established for farming Chanos chanos in southwestern Taiwan. In this system, fishes are cultured in combination with organic extractive shellfish and inorganic extractive seaweed. This study aimed to evaluate the prevalence of sulfonamide-resistant bacteria, microbial community structure, and occurrence of sulfonamide resistance genes in the IMTA and traditional aquaculture systems. Water and sediment samples were collected before raising and after harvesting C. chanos. Our results showed that the occurrence of sulfonamide-resistant phenotypes in the IMTA system was comparable with that in influent seawater, while the traditional system exhibited a high sulfonamide resistance rate. Additionally, the traditional system resulted in a deviation of the bacterial community structure from that of seawater. In the water samples from the IMTA system and influent seawater, Proteobacteria and Bacteroidetes were the two dominant phyla, representing approximately 75% and 15% of the community, respectively. In the traditional system, Actinobacteria, constituting 39% of the community, was the dominant bacterial phylum. Thirty-one sulfonamide-resistant bacterial species were isolated. In conclusion, a sequentially IMTA system showed superior ability to maintain the prevalence of antibiotic resistance and the integrity of the bacterial community structure compared to the traditional farming system, representing a potentially valuable aquaculture system for preserving the sustainability of the marine environment. © 2018
Subjects
Antibiotic resistance; Chanos chanos; Integrated multi-trophic aquaculture; Microbial community composition
SDGs
Other Subjects
Antibiotics; Aquaculture; Bacteria; Genes; Seawater; Sustainable development; Antibiotic resistance; Aquaculture systems; Bacterial community structure; Chanos chanos; Environment friendly; Microbial community composition; Microbial community structures; Organic extractives; Sulfur compounds; sea water; sulfonamide; sulfonamide; antibiotic resistance; aquaculture; bacterium; community composition; community structure; fish; gene; microbial community; Actinobacteria; aquaculture; Article; bacterium; bacterium isolate; Bacteroidetes; Chanos chanos; community structure; gene; microbial community; multi trophic aquaculture system; nonhuman; phenotype; phylum; prevalence; priority journal; Proteobacteria; seaweed; sediment; shellfish; sul1 gene; sulfonamide resistant bacterium; sustainable agriculture; water sampling; agricultural land; animal; antibiotic resistance; bacterial gene; bacterium; environmental monitoring; genetics; microbiology; prevalence; Taiwan; Taiwan; Actinobacteria; Bacteria (microorganisms); Bacteroidetes; Chanos chanos; Pisces; Proteobacteria; Animals; Aquaculture; Bacteria; Drug Resistance, Bacterial; Environmental Monitoring; Farms; Genes, Bacterial; Prevalence; Sulfonamides; Taiwan; Water Microbiology
Type
journal article