Quantitative analysis of multiphoton excitation autofluorescence and second harmonic generation imaging for medical diagnosis
Journal
Computerized Medical Imaging and Graphics
Journal Volume
36
Journal Issue
7
Pages
519-526
Date Issued
2012
Author(s)
Abstract
In recent years, two-photon excitation fluorescence and second harmonic generation microscopy has become an important tool in biomedical research. The ability of two-photon microscopy to achieve optical sectioning with minimal invasiveness is particularly advantageous for biomedical diagnosis. Advances in the miniaturization of the imaging system have increased its clinical potential, together with the development of quantitative technique for the analysis of data acquired using these imaging modalities. We present a review of the quantitative analysis techniques that have been used successfully with two-photon excitation fluorescence and SHG imaging. Specifically, quantification techniques using ratiometric, morphological, and structural differences to analyze two-photon images will be discussed, and their effectiveness at evaluating dermal and corneal pathologies and cancerous tumor growth will be described. ? 2012 Elsevier Ltd.
Subjects
Image analysis; Nonlinear optics; Optical microscopy; Second harmonic generation; Two-photon
SDGs
Other Subjects
Analysis of data; Autofluorescence; Biomedical research; Cancerous tumors; Corneal pathologies; Imaging modality; Invasiveness; Multiphoton excitation; Optical sectioning; Quantitative techniques; Ratiometric; Second harmonic generation microscopies (SHG); Second harmonics; Structural differences; Two photon; Two photon excitation fluorescence; Two photon microscopy; Biomedical engineering; Diagnosis; Fluorescence; Harmonic generation; Image analysis; Optical microscopy; Photons; Nonlinear optics; autofluorescence imaging; basal cell carcinoma; cancer growth; cornea disease; elastolysis; electronics; fluorescence microscopy; Fourier transformation; human; keloid; lung adenocarcinoma; lung squamous cell carcinoma; medical research; minimally invasive procedure; morphea; multiphoton microscopy; nonhuman; optical coherence tomography; priority journal; quantitative analysis; review; second harmonic generation; Equipment Design; Evaluation Studies as Topic; Image Processing, Computer-Assisted; Microscopy, Fluorescence, Multiphoton; Photons
Type
other