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  4. Spatial and temporal analysis of skin glycation by the use of multiphoton microscopy and spectroscopy
 
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Spatial and temporal analysis of skin glycation by the use of multiphoton microscopy and spectroscopy

Journal
Journal of Dermatological Science
Journal Volume
65
Journal Issue
3
Pages
189-195
Date Issued
2012
Author(s)
Ghazaryan, A.A.
Hu, P.-S.
Chen, S.-J.
Tan, H.-Y.
CHEN-YUAN DONG  
DOI
10.1016/j.jdermsci.2011.12.012
URI
https://scholars.lib.ntu.edu.tw/handle/123456789/436325
URL
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84862783107&doi=10.1016%2fj.jdermsci.2011.12.012&partnerID=40&md5=59a391dda4a73e162491e3c5ff594916
Abstract
Background: Tissue glycation, the main cause of many diabetes-related complications, results in the accumulation of advanced glycation endproducts (AGE). Objectives: These AGEs are endogenous fluorophores that can serve as a viable pathological indicator for disease diagnostics. Here we explore the capabilities of multiphoton microscopy to non-invasively localize and quantify the skin glycation. Methods: In our study, multiphoton microscopy and spectroscopy were used to investigate glycation events-induced changes in the intensities of autofluorescence and second harmonic generation on ex vivo human skin. Results: Temporal and spatial dependence of degrees of glycation of the epidermis, collagen and elastin fibers of dermis were evaluated for their relevance to the changes in amplitudes of autofluorescence signals. We found that glycation drastically and linearly increases multiphoton autofluorescence intensity of epidermis and dermal collagen whereas changes in dermal elastin are moderate. We also found decrease in the level of second harmonic generation signal. Conclusion: Our study suggests that due to intrinsically weak autofluorescence the dermal collagen is the most sensitive skin tissue to be used for detecting changes in tissue glycation. ? 2012 Japanese Society for Investigative Dermatology.
Subjects
Advanced glycation endproducts; Multiphoton imaging; Second harmonic generation; Tissue glycation
SDGs

[SDGs]SDG3

Other Subjects
advanced glycation end product; collagen fiber; elastin; article; autofluorescence; controlled study; epidermis; ex vivo study; glycation; human; human tissue; multiphoton microscopy; priority journal; signal processing; skin glycation; spectroscopy; tissue glycation; Cadaver; Collagen; Elastic Tissue; Epidermis; Glycosylation End Products, Advanced; Humans; Male; Microscopy, Fluorescence, Multiphoton; Middle Aged; Skin; Spectrometry, Fluorescence
Type
journal article

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