https://scholars.lib.ntu.edu.tw/handle/123456789/436437
標題: | Enhanced anticancer effects of low-dose curcumin with non-invasive pulsed electric field on PANC-1 cells | 作者: | Lu, C.-H. Lin, S.-H. Hsieh, C.-H. Chen, W.-T. CHIH-YU CHAO |
關鍵字: | Combination treatment; Curcumin; Pancre-atic cancer; Pulsed electric field; Synergistic effect | 公開日期: | 2018 | 卷: | 11 | 起(迄)頁: | 4723-4732 | 來源出版物: | OncoTargets and Therapy | 摘要: | Background: Pulsed electric field (PEF) has been considered as a cell permeability enhancing agent for cancer treatment. Nevertheless, application of PEF for conventional electrochemotherapy is usually at high intensity, and contact or even invasive electrodes are typically used, which may cause unwanted side effects. In this study, a non-invasive way of applying low intensity, non-contact PEF was adopted to study its combination effect with herb, curcumin, against pancreatic cancer cells and the mechanism involved. Methods: The pancreatic cancer PANC-1 cells were treated with curcumin and PEF alone or in combination, and MTT assay was used to determine the viability of PANC-1 cells. Apoptosis and uptake of curcumin were analyzed by microscopy and flow cytometry. Western blot was further performed to evaluate the expression of apoptotic proteins. Results: Our results demonstrated that PEF synergized with curcumin to inhibit the proliferation of PANC-1 cells in a field strength-and dose-dependent manner and caused apoptotic death of PANC-1 cells. The apoptotic induction of combination treatment was characterized by an increase in Bax/Bcl-2 ratio, and cleavage of caspase-8,-9, and-3. Moreover, the increase of curcumin uptake via electro-endocytosis was clearly observed in the cells following the exposure of PEF. Conclusion: We show for the first time that a non-contact approach using low intensity electric field in a pulsed waveform could enhance the anticancer effect of low-dose curcumin on PANC-1 cells through triggering both extrinsic and intrinsic pathways. The findings highlight the potential of this alternative treatment, non-invasive electric field and curcumin, to increase therapeutic efficacy with minimum cytotoxicity and side effects, which may provide a new aspect of cancer treatment in combination of PEF and other anticancer agents. ? 2018 Lu et al. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/436437 | DOI: | 10.2147/OTT.S166264 | SDG/關鍵字: | apoptosis regulatory protein; caspase 3; caspase 8; caspase 9; curcumin; protein Bax; protein bcl 2; alternative medicine; antineoplastic activity; antiproliferative activity; apoptosis; Article; cancer therapy; cell proliferation; cell viability; controlled study; dose response; drug cytotoxicity; drug efficacy; drug mechanism; drug potentiation; endocytosis; flow cytometry; human; human cell; low drug dose; microscopy; monotherapy; MTT assay; non invasive procedure; PANC-1 cell line; pancreatic cancer cell line; protein cleavage; protein expression; pulsed electric field; signal transduction; strength; therapy effect; waveform; Western blotting |
顯示於: | 物理學系 |
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