Electrochemical β(1→3)-d-glucan biosensors fabricated by immobilization of enzymes with gold nanoparticles on platinum electrode
Journal
Biosensors and Bioelectronics
Journal Volume
26
Journal Issue
1
Pages
118-125
Date Issued
2010
Author(s)
Abstract
β(1→3)-d-Glucan sensors were fabricated using bi-enzyme and tri-enzyme immobilized systems with gold nanoparticles (GNPs) to increase sensitivity. The plant β(1→3)-d-glucanase (βG), glucose oxidase (GOD) or/and peroxidase (POD) in agarose-corn flour-gelatin (ACG) matrix were coated on platinum disc electrode to detect soluble β(1→3)-d-glucan. The atomic force microscopy (AFM) revealed that GNPs embedded in ACG formed tiny islands/clusters with enzymes. Both of bi-enzyme sensor (ACG-βG-GOD-GNPs/Pt) and tri-enzyme sensor (ACG-βG-GOD-POD-GNPs/Pt) had response time less than 20s for β(1→3)-d-glucan. A linear calibration plot for bi-enzyme sensor was obtained for β(1→3)-d-glucan concentration ranged from 100 to 1000ngmL-1 (R2=0.983). The lower detection limit was 30ngmL-1 using applied potential of 200mV and scan rate of 50mVs-1; with signal to noise ratio (S/N) of 3. Fabricated tri-enzyme sensor was also operable under similar conditions with LOD of 50ngmL-1 (R2=0.989) at -175mV applied potential and scan rate of 50mVs-1. Both sensors were durable and could be repeatedly used for at least 14 times. When the tri-enzyme sensor was employed to analyze β(1→3)-d-glucan content in alcoholic beverages, the results were comparable to those obtained by standard method. © 2010 Elsevier B.V.
SDGs
Type
journal article