https://scholars.lib.ntu.edu.tw/handle/123456789/438686
標題: | A Mechatronic Microinjection Platform for Gene Delivery to Planarian | 作者: | Lee M. CHIA-YING CHU YEN-WEN LU |
公開日期: | 2018 | 出版社: | Institute of Electrical and Electronics Engineers Inc. | 起(迄)頁: | Article number 8519991 | 來源出版物: | 2018 International Conference on System Science and Engineering, ICSSE 2018 | 摘要: | We presents a microinjection system for gene delivery to planarian. The system consists of two parts: a planarian-fixing platform, which provides a low-temperature environment to make planarian become motionless for injection, and a nanoliter injector (nanoliter-scale microinjector). The system is tested by injecting fluorescent microbeads and gene (e.g. dsRNA) respectively into planarian. In particular, successful delivery of DjAg02 dsRNA leads to a reduction of DjAg02 expression, which results in a degenerated phenotype of planarian. The results show our system as an alternative to traditional gene delivery (i.e. ingestion, which performs delivery by feeding planarian genetic substances mixed food). Our system is applicable to studying the function of genes related to starvation condition. Additionally, the dosage of genetic substances is consistent, which is required for the following analyses. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/438686 | ISBN: | 9781538662854 | DOI: | 10.1109/ICSSE.2018.8519991 |
顯示於: | 生物機電工程學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。