A Mechatronic Microinjection Platform for Gene Delivery to Planarian
Journal
2018 International Conference on System Science and Engineering, ICSSE 2018
Pages
Article number 8519991
ISBN
9781538662854
Date Issued
2018
Author(s)
Abstract
We presents a microinjection system for gene delivery to planarian. The system consists of two parts: a planarian-fixing platform, which provides a low-temperature environment to make planarian become motionless for injection, and a nanoliter injector (nanoliter-scale microinjector). The system is tested by injecting fluorescent microbeads and gene (e.g. dsRNA) respectively into planarian. In particular, successful delivery of DjAg02 dsRNA leads to a reduction of DjAg02 expression, which results in a degenerated phenotype of planarian. The results show our system as an alternative to traditional gene delivery (i.e. ingestion, which performs delivery by feeding planarian genetic substances mixed food). Our system is applicable to studying the function of genes related to starvation condition. Additionally, the dosage of genetic substances is consistent, which is required for the following analyses.
Publisher
Institute of Electrical and Electronics Engineers Inc.
Type
conference paper