https://scholars.lib.ntu.edu.tw/handle/123456789/441790
標題: | Spectral ophthalmoscopy based on supercontinuum | 作者: | Cheng, Y.-H. Yu, J.-Y. Wu, H.-H. Huang, B.-J. Chu, S.-W. SHI-WEI CHU |
關鍵字: | Confocal scanning laser ophthalcoscope; Off-axis aberration; Optical sectioning; Retina; Zebrafish | 公開日期: | 2010 | 卷: | 7568 | 來源出版物: | Progress in Biomedical Optics and Imaging - Proceedings of SPIE | 摘要: | Confocal scanning laser ophthalmoscope (CSLO) has been established to be an important diagnostic tool for retinopathies like age-related macular degeneration, glaucoma and diabetes. Compared to a confocal laser scanning microscope, CSLO is also capable of providing optical sectioning on retina with the aid of a pinhole, but the microscope objective is replaced by the optics of eye. Since optical spectrum is the fingerprint of local chemical composition, it is attractive to incorporate spectral acquisition into CSLO. However, due to the limitation of laser bandwidth and chromatic/geometric aberration, the scanning systems in current CSLO are not compatible with spectral imaging. Here we demonstrate a spectral CSLO by combining a diffraction-limited broadband scanning system and a supercontinuum laser source. Both optical sectioning capability and sub-cellular resolution are demonstrated on zebrafish's retina. To our knowledge, it is also the first time that CSLO is applied onto the study of fish vision. The versatile spectral CSLO system will be useful to retinopathy diagnosis and neuroscience research. ? 2010 Copyright SPIE - The International Society for Optical Engineering. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/441790 | DOI: | 10.1117/12.843020 | SDG/關鍵字: | Confocal scanning; Off-axis; Off-axis aberration; Optical sectioning; Zebrafish; Aberrations; Biomolecules; Histology; Imaging systems; Light; Scanning |
顯示於: | 物理學系 |
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