Coherent interaction of optical second harmonic generation in collagen fibrils
Journal
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Journal Volume
6860
Date Issued
2008
Author(s)
Chu, S.-W.
Tai, S.-P.
Chan, M.-C.
Sun, C.-K.
Hsiao, I.-C.
Lin, C.-H.
Chen, Y.-C.
Abstract
We present the first experimental comparison between optical second harmonic generation images and atomic force microscope images in a matrix of nano-scaled collagen fibrils. Substantial variation of forward and backward propagated second harmonic generation radiation is observed in a single collagen fibril and is nicely correlated with the accurately determined thickness from an atomic force microscope. Contradicting to conventional nonlinear optical theory, our result indicates a linear relationship between fibril thickness and forward / backward second harmonic generation ratio. This is the first demonstration of estimating fibril thickness with nanometer precision by a noninvasive optical method.
Type
conference paper