Invitro Complex-Formation Between Bacteriophage Phi-29-Terminal Protein and Deoxynucleotide
Journal
Biochemical and Biophysical Research Communications
Journal Volume
105
Journal Issue
3
Pages
1031-1036
Date Issued
1982
Author(s)
Abstract
It has previously been shown that the 5′-terminal deoxyadenosine residue of each φ29 DNA strand is linked covalently to the 30,000 dalton terminal protein. When extracts prepared from φ29-infectedBacillussubtilis cells are incubated with [α-32p]dATP, complexes consisting of the nucleotide covalently linked to a 30,000 dalton protein can be detected. The formation of this complex requires the presence of φ29 DNA containing the bound 30,000 dalton terminal protein and Mg++. When uninfected cell extracts were used, there was no complex formation. When [α-32p]dCTP was used in place of [α-32p]dATP, no complex was formed. DNA-protein templates prepared from φ29 related phages, φ15, Nf, M2Y and GA-1, also supported the complex formation in various degrees. These results support the hypothesis that the terminal protein serves as a primer for the initiation of φ29 DNA replication. © 1982.
Type
journal article