Areca nut extracts enhance the development of CD11b(+) Gr-1(+) cells with the characteristics of myeloid-derived suppressor cells in antigen-stimulated mice
Journal
Journal of Oral Pathology & Medicine
Journal Volume
40
Journal Issue
10
Pages
769-777
Date Issued
2011
Author(s)
Abstract
Background: Areca quid chewing is an etiological factor contributing to the development of oral cancer and pre-cancers, whose pathophysiology has been linked to inflammation and immune deterioration. Myeloid-derived suppressor cells (MDSC) play a key role in the regulation of immunity under certain pathological conditions, such as inflammation and cancer. As areca nut extracts (ANE) have been reported to induce a proinflammatory effect in antigen-stimulated mice, we hypothesized that ANE might enhance the development of MDSC. Methods: Ovalbumin (OVA)-sensitized BALB/c mice were daily administered with ANE (5-50mg/kg), polyphenol-enriched ANE (PANE; 25mg/kg) or arecoline (5mg/kg) by intraperitoneal injection for 10 doses. The mouse footpads were then subcutaneously challenged with OVA to induce local inflammatory responses. Results: ANE and PANE treatment significantly increased the spleen index and the population of CD11b +Gr-1 + cells in the spleen and peripheral blood, whereas arecoline was inactive. In addition, ANE and PANE treatment enhanced the expression of cytokines and enzymes associated with the immunosuppressive function of MDSC, including IL-10, arginase-I and iNOS in splenic CD11b + cells. Concordantly, ANE and PANE treatment augmented the infiltration of Gr-1 +IL-10 + cells in the footpads challenged with OVA. Conclusions: Our results suggested that areca nut constituents, in particular, polyphenols enhanced the development of myeloid-derived suppressor cells in vivo, which may be a critical mechanism linking inflammation and the compromised immunity reported to be associated with the pathophysiology of areca-related oral diseases. ? 2011 John Wiley & Sons A/S.
SDGs
Other Subjects
areca nut extract; arecoline; arginase 1; CD11b antigen; cytokine; enzyme; inducible nitric oxide synthase; interleukin 10; ovalbumin; plant extract; polyphenol; unclassified drug; animal experiment; article; Bagg albino mouse; betel nut; blood; bone marrow cell; cell function; cell infiltration; cell maturation; controlled study; foot pad; immunostimulation; inflammation; male; mouse; nonhuman; priority journal; protein expression; spleen; suppressor cell; Animals; Antigens, CD11b; Areca; Arecoline; Arginase; Body Weight; Cell Culture Techniques; Chemotaxis, Leukocyte; Cholinergic Agonists; Immune Tolerance; Immunization; Inflammation Mediators; Interleukin-10; Leukocytes, Mononuclear; Male; Mice; Mice, Inbred BALB C; Monocytes; Myeloid Cells; Nitric Oxide Synthase Type II; Nuts; Organ Size; Ovalbumin; Plant Extracts; Polyphenols; Receptors, Chemokine; Spleen
Type
journal article