Cryopreservation of a marine microalga, Nannochloropsis oculata (Eustigmatophyceae)
Journal
Cryobiology
Journal Volume
50
Journal Issue
3
Pages
338-343
Date Issued
2005
Author(s)
Abstract
The cryopreservation of algae could prevent genetic drift and minimize labor costs compared to the current method of maintenance and subculturing. Clear, simple protocols for cryopreservation of marine microalga, Nannochloropsis oculata were developed and cryoprotectant choice and concentration optimized. The viability of the microalga was assessed directly after thawing, and algal concentration was measured after 2-30 days of growth. Five cryoprotectants (dimethyl sulphoxide, Me2SO; ethylene glycol, EG; glycerol, Gly; methanol, MeOH; and propylene glycol, PG) at five concentrations (10, 20, 30, 40, and 50%; v/v) were evaluated to determine the toxicity of various cryoprotectants to N. oculata. The toxicity of cryoprotectant (Me2SO, EG, MeOH, and PG) was observed only at higher concentrations of CPAs: >20% for EG, >30% for Me2SO and methanol, and >40% for PG. Direct freezing of algae in liquid nitrogen resulted in a severe loss of viability and a modified cryopreservation protocol proved to be more appropriate for the preservation of N. oculata. Cryopreservation protocols developed and tested in the present study might be applied to cryopreserving other strains, or species, in this genus. © 2005 Elsevier Inc. All rights reserved.
Subjects
Algae; Aquaculture; Feed; Freezing; Morphology; Nannochloropsis oculata
SDGs
Other Subjects
cryoprotective agent; dimethyl sulfoxide; ethylene glycol; glycerol; methanol; nitrogen; propylene glycol; alga; algal growth; article; cell viability; concentration (parameters); cryopreservation; freezing; liquid; marine environment; nonhuman; priority journal; species identification; thawing; toxicity testing; Algae; Animals; Cryopreservation; Cryoprotective Agents; Dimethyl Sulfoxide; Ethylene Glycol; Glycerol; Marine Biology; Methanol; Propylene Glycol; algae; Eustigmatophyceae; Nannochloropsis oculata
Type
journal article