Honokiol, a polyphenol natural compound, attenuates cisplatin-induced acute cytotoxicity in renal epithelial cells through cellular oxidative stress and cytoskeleton modulations
Journal
Frontiers in Pharmacology
Journal Volume
9
Journal Issue
APR
Date Issued
2018
Author(s)
Wang T.J.
Liu H.-T.
Lai Y.-H.
Jan T.-R.
Nomura N.
Chang H.-W.
Chou C.-C.
Lee Y.-J.
Abstract
Cisplatin is a potent anti-cancer drug that has been widely used in the treatment of various cancers; however, cisplatin administration results in severe nephrotoxicity and impedes its clinical applications. In this study, we showed that honokiol, a polyphenol constituent extracted from Magnolia officinalis exhibited a short-term protective effect against cisplatin-induced damages in renal epithelial cells in vitro. The protective effects of honokiol were resulted from the combination of (1) reduced cellular oxidative stress ranging from 53 to 32% reduction during a 24-h incubation, (2) the maintenance of cellular antioxidant capacity and (3) the stabilization of cytoskeletal structure of the kidney epithelial cells. By promoting the polymerization of actin (1.6-fold increase) and tubulin (1.8-fold increase) cytoskeleton, honokiol not only maintained epithelial cell morphology, but also stabilized cellular localizations of tight junction protein Occludin and adhesion junction protein E-Cadherin. With stabilized junction protein complexes and structural polymerized cytoskeleton network, honokiol preserved epithelial cell polarity and morphology and thus reduced cisplatin-induced cell disruption and damages. Our data demonstrated for the first time that honokiol could counteract with cisplatin-induced damages in renal epithelial cells in vitro, future in vivo studies would further validate the potential clinical application of honokiol in cisplatin-based cancer treatments with reduced nephrotoxicity. ? 2018 Wang, Liu, Lai, Jan, Nomura, Chang, Chou, Lee and Tsai.
SDGs
Other Subjects
actin antibody; cisplatin; honokiol; Magnolia officinalis extract; occludin; tubulin; uvomorulin; actin polymerization; animal cell; antioxidant assay; Article; cell culture; cell damage; cell disruption; cell protection; cell structure; cellular distribution; controlled study; cytoskeleton; cytotoxicity; epithelium cell; in vitro study; kidney epithelium; Magnolia officinalis; nephrotoxicity; nonhuman; occluding junction; oxidative stress
Type
journal article