https://scholars.lib.ntu.edu.tw/handle/123456789/444592
標題: | Iron oxide nanoparticles attenuate T helper 17 cell responses in vitro and in vivo | 作者: | Hsiao Y.-P. Shen C.-C. Huang C.-H. Lin Y.-C. TONG-RONG JAN |
關鍵字: | Delayed-type hypersensitivity; Iron oxide nanoparticle; Ovalbumin; Splenocytes; Th17 | 公開日期: | 2018 | 卷: | 58 | 起(迄)頁: | 32-39 | 來源出版物: | International Immunopharmacology | 摘要: | Iron oxide nanoparticles (IONPs) have been shown to attenuate T helper (Th)1 and Th2 cell-mediated immunity in ovalbumin (OVA)-sensitized mice. The objective of this study is to investigate the effects of IONPs on the immune responses of Th17 cells, a subset of T cells involved in various inflammatory pathologies. For in vivo study, a murine model of delayed-type hypersensitivity (DTH) was employed. BALB/c mice received a single dose of IONPs (0.2–10 mg iron/kg) via the tail vein 1 h prior to ovalbumin (OVA) sensitization. Their footpads were subcutaneously challenged with OVA to induce DTH reactions. The expression of Th17 cell-related molecules in inflamed footpads were examined by immunohistochemistry. For in vitro study, OVA-primed splenocytes were directly exposed to IONPs (1–100 μg iron/mL), and then re-stimulated with OVA in culture. The expression of Th17 cell-related molecules were measured by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. IONP administration attenuated the number of interleukin (IL)-6, IL-17, the transcription factor ROR-γ and chemokine receptor 6 positive cells in OVA-challenged footpads, whereas the number of transforming growth factor-β IL-23 and chemokine (C-C motif) ligand 20 positive cells was not altered. Direct exposure of OVA-primed splenocytes to IONPs suppressed the production of IL-6 and IL-17, and the mRNA expression of IL-17 and ROR-γt. These data indicate that exposure to IONPs attenuates Th17 cell responses in vivo and in vitro. ? 2018 |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/444592 | ISSN: | 1567-5769 | DOI: | 10.1016/j.intimp.2018.03.007 | SDG/關鍵字: | chemokine receptor; ferucarbotran; interleukin 17; interleukin 23; interleukin 6; ovalbumin; retinoid related orphan receptor gamma; transforming growth factor beta; ultrasmall superparamagnetic iron oxide; allergen; cytokine; ferric ion; ferric oxide; immunosuppressive agent; nanoparticle; retinoid related orphan receptor gamma; animal cell; animal experiment; animal model; animal tissue; Article; cellular immunity; controlled study; delayed hypersensitivity; foot pad; immunohistochemistry; immunomodulation; in vitro study; in vivo study; male; mouse; nonhuman; priority journal; protein expression; sensitization; spleen cell; Th17 cell; Th2 cell; animal; Bagg albino mouse; cell culture; delayed hypersensitivity; disease model; drug effect; human; immunology; metabolism; T lymphocyte subpopulation; Th17 cell; Allergens; Animals; Cells, Cultured; Cytokines; Disease Models, Animal; Ferric Compounds; Humans; Hypersensitivity, Delayed; Immunosuppressive Agents; Male; Mice; Mice, Inbred BALB C; Nanoparticles; Nuclear Receptor Subfamily 1, Group F, Member 3; Ovalbumin; T-Lymphocyte Subsets; Th17 Cells |
顯示於: | 獸醫學系 |
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