Inhibition of urease activity by humic acid extracted from sludge fermentation liquid
Journal
Bioresource Technology
Journal Volume
290
Date Issued
2019
Author(s)
Abstract
This study achieved effective extraction of humic acid from sludge fermentation liquid, and the inhibition of urease activity by the extract were investigated in the urea decomposition. The addition of extract could remarkably inhibit urease activity and extend the releasing time of ammonia nitrogen. The interaction between the extract and urease took times, and the inhibition was irreversible. The results of fluorescence analysis revealed that the inhibition of urease activity was correlated to the amount of humic acid extracted. The mechanisms of inhibition were proposed that the functional groups of humic acid might interact with the thiol group of urease and formed a larger particle size of complex to inhibit the activity of urease. The extraction of humic acid from sludge fermentation liquid can not only recover the resource from the fermentation liquid, but also provide a potential urease inhibitor for the sustained-release effect of the soil organic nitrogen fertilizer. © 2019 Elsevier Ltd
Subjects
Humic acid; Inhibition; Sludge fermentation liquid; Sustained-release effect; Urease activity
SDGs
Other Subjects
Ammonia; Biological materials; Enzyme inhibition; Fermentation; Fluorescence; Liquids; Nitrogen fertilizers; Organic acids; Particle size; Particle size analysis; Urea; Fluorescence analysis; Humic acid; Larger particle sizes; Sludge fermentation; Sustained release; Urea decomposition; Urease activity; Urease inhibitors; Extraction; humic acid; nitrogen; organic nitrogen; thiol group; urea; urease; fertilizer; urease; activated sludge; enzyme activity; extraction method; fermentation; humic acid; inhibition; liquid; Article; decomposition; enzyme activity; extraction; fermentation; fluorescence analysis; liquid; particle size; priority journal; sludge; fermentation; humic substance; sewage; Fermentation; Fertilizers; Humic Substances; Sewage; Urease
Type
journal article