https://scholars.lib.ntu.edu.tw/handle/123456789/446061
標題: | The urinary shedding of porcine teschovirus in endemic field situations | 作者: | Tsai, Arthur Tung-Hsuan Kuo, Chien-Chun Kuo, Yi-Ching Yang, Jia-Ling CHIA-YI CHANG FUN-IN WANG |
關鍵字: | Molecular serotyping; Nasal swab; Pathogenesis; Plasma; Porcine teschovirus; QRT-PCR; Urine; Viral load | 公開日期: | 2016 | 卷: | 182 | 起(迄)頁: | 150-155 | 來源出版物: | Veterinary Microbiology | 摘要: | Porcine teschoviruses (PTVs) belong to the genus Teschovirus within the family Picornaviridae. PTVs are universal contaminants in pig herds in endemic and multi-infection statuses. Previous research has demonstrated PTV antigens and nucleic acid in renal glomeruli and tubular epithelia, suggesting the possibility that PTVs might be shed and transmitted via urine. The study aimed to demonstrate, in the context of pathogenesis, the presence of PTVs in the urine of naturally infected pigs. Viral loads of fluid and tissue samples quantified by an established qRT-PCR showed detection rates of 100% by head and in urine, feces, plasma and nasal swabs, and 38% in kidney. As predicted, PTVs were present in urine at 104.02±1.45 copies/100μl volume, equivalent to 17% of that in plasma. No significant differences were observed between healthy and culled pigs or among the 7 sampled herds. The presence of PTVs in urine was further substantiated by molecular serotyping. In particular, PTV-10 was identified in the urine of 3 piglets from 3 separate herds, consistent with the most prevalent serotype found in this study, and in plasma. The urine mixes with feces to form slurry making it easier for PTV to spread and contaminate the environment. ? 2015 Elsevier B.V.. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/446061 | ISSN: | 03781135 | DOI: | 10.1016/j.vetmic.2015.11.008 | SDG/關鍵字: | controlled study; feces; head; herd; human; human tissue; nose smear; pathogenesis; piglet; plasma; polymerase chain reaction; quantitative study; serotype; serotyping; Teschovirus; urine; virus load; animal; endemic disease; genetics; isolation and purification; physiology; picornavirus infection; pig; swine disease; Teschovirus; transmission; veterinary; virology; virus shedding; Animals; Endemic Diseases; Picornaviridae Infections; Serogroup; Sus scrofa; Swine; Swine Diseases; Teschovirus; Urine; Viral Load; Virus Shedding |
顯示於: | 獸醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。