Display of porcine epidemic diarrhea virus spike protein on baculovirus to improve immunogenicity and protective efficacy
Journal
Viruses
Journal Volume
10
Journal Issue
7
Date Issued
2018
Author(s)
Abstract
A new variant of the porcine epidemic diarrhea virus (PEDV) is an emerging swine disease, killing considerable numbers of neonatal piglets in North America and Asia in recent years. To generate immunogens mimicking the complex spike (S) protein folding with proper posttranslational modification to mount a robust immune response against the highly virulent PEDV, two baculoviruses displaying the full-length S protein (S-Bac) and the S1 protein (S1-Bac) of the virulent Taiwan genotype 2b (G2b) PEDV Pintung 52 (PEDV-PT) strain were constructed. Intramuscular immunizations of mice and piglets with the S-Bac and S1-Bac demonstrated significantly higher levels of systemic anti-PEDV S-specific IgG, as compared with control group. Our results also showed that piglets in the S-Bac group elicited superior PEDV-specific neutralizing antibodies than those of the S1-Bac and control groups. The highly virulent PEDV-PT strain challenge experiment showed that piglets immunized with S-Bac and S1-Bac showed milder clinical symptoms with significantly less fecal viral shedding as compared with non-immunized control piglets. More importantly, piglets immunized with the S-Bac exhibited no to mild clinical signs, with a delayed, minimal viral shedding. Our results demonstrated that the S-Bac could serve as a safe, easy to manipulate, and effective vaccine candidate against the PEDV infection. ? 2018 by the authors. Licensee MDPI, Basel, Switzerland.
Subjects
Baculovirus display system; Intramuscular injection; PEDV; Spike vaccine
SDGs
Other Subjects
immunoglobulin A; immunoglobulin G; neutralizing antibody; porcine epidemic diarrhea virus S protein; porcine epidemic diarrhea virus S1 protein; unclassified drug; virus spike protein; coronavirus spike glycoprotein; immunoglobulin G; virus antibody; virus vaccine; animal cell; animal experiment; Article; Baculoviridae; body weight; controlled study; diarrhea; electron microscopy; enzyme linked immunosorbent assay; feces analysis; human; human cell; immune response; immunization; immunogenicity; mouse; nonhuman; nucleotide sequence; piglet; Porcine epidemic diarrhea virus; protein expression; protein processing; real time polymerase chain reaction; scoring system; virus neutralization; virus particle; virus recombinant; virus shedding; animal; Baculoviridae; blood; Coronavirus infection; feces; genetics; genotype; immunology; intramuscular drug administration; pig; Porcine epidemic diarrhea virus; suckling animal; swine disease; vaccine immunogenicity; virology; Animals; Animals, Suckling; Antibodies, Neutralizing; Antibodies, Viral; Baculoviridae; Coronavirus Infections; Feces; Genotype; Immunogenicity, Vaccine; Immunoglobulin G; Injections, Intramuscular; Porcine epidemic diarrhea virus; Spike Glycoprotein, Coronavirus; Swine; Swine Diseases; Viral Vaccines; Virus Shedding
Type
journal article