Rapid differentiation between short-chain-length and medium-chain-length polyhydroxyalkanoate-accumulating bacteria with spectrofluorometry
Journal
Journal of Microbiological Methods
Journal Volume
53
Journal Volume
53
Journal Issue
1
Journal Issue
1
Pages
131-135
Start Page
131
End Page
135
ISSN
01677012
Date Issued
2003-04-01
Author(s)
Abstract
An approach for rapid differentiation between short-chain-length (scl) and medium-chain-length (mcl) polyhydroxyalkanoate (PHA) producers was developed. Polyhydroxyalkanoate-accumulated bacterial cells stained with Nile red were suspended in water and subjected to fluorescence spectroscopy at a fixed excitation wavelength of 488 nm. The scl-PHA-accumulated bacteria revealed a maximum emission wavelength at 590 nm, and for mcl-PHA producers were seen at a wavelength of 575 nm. Combining Nile red staining and fluorescence spectroscopy, the accumulated PHA granules could be rapidly differentiated into scl-PHA and mcl-PHA from the intact cells.
Subjects
Fluorescence spectroscopy
Medium-chain-length
Nile red
Polyhydroxyalkanoate
Short-chain-length
SDGs
Type
journal article