https://scholars.lib.ntu.edu.tw/handle/123456789/446201
標題: | Degradation of polyurethane by bacterium isolated from soil and assessment of polyurethanolytic activity of a Pseudomonas putida strain | 作者: | Peng Y.-H. YANG-HSIN SHIH Lai Y.-C. Liu Y.-Z. Liu Y.-T. NAI-CHUN LIN |
公開日期: | 2014 | 卷: | 21 | 期: | 16 | 起(迄)頁: | 9529-9537 | 來源出版物: | Environmental Science and Pollution Research | 摘要: | The increasing usage and the persistence of polyester polyurethane (PU) generate significant sources of environmental pollution. The effective and environmental friendly bioremediation techniques for this refractory waste are in high demand. In this study, three novel PU degrading bacteria were isolated from farm soils and activated sludge. Based upon 16S ribosomal RNA gene sequence blast, their identities were determined. Particularly robust activity was observed in Pseudomonas putida; it spent 4 days to degrade 92 % of Impranil DLNTM for supporting its growth. The optimum temperature and pH for DLN removal by P. putida were 25 °C and 8.4, respectively. The degradation and transformation of DLN investigated by Fourier transformed infrared spectroscopy show the decrease in ester functional group and the emergence of amide group. The polyurethanolytic activities were both presented in the extracellular fraction and in the cytosol. Esterase activity was detected in the cell lysate. A 45-kDa protein bearing polyurethanolytic activity was also detected in the extracellular medium. This study presented high PU degrading activity of P. putida and demonstrated its responsible enzymes during the PU degradation process, which could be applied in the bioremediation and management of plastic wastes. © 2014 Springer-Verlag Berlin Heidelberg. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/446201 | ISSN: | 09441344 | DOI: | 10.1007/s11356-014-2647-8 | SDG/關鍵字: | activated sludge; bacterium; biodegradation; bioremediation; ester; functional group; gene; protein; RNA; Bacteria (microorganisms); Pseudomonas putida; polyester; polyurethan; sewage; bioremediation; chemistry; metabolism; microbiology; Pseudomonas putida; sewage; Biodegradation, Environmental; Polyesters; Polyurethanes; Pseudomonas putida; Sewage; Soil Microbiology |
顯示於: | 農業化學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。