https://scholars.lib.ntu.edu.tw/handle/123456789/446228
Title: | Soy isoflavone-phosphate conjugates derived by cultivating Bacillus subtilis var. natto BCRC 80517 with isoflavone | Authors: | Hsu C. Ho H.-W. Chang C.-F. Wang S.-T. Fang T.-F. Lee M.-H. NAN-WEI SU NAN-WEI SU |
Issue Date: | 2013 | Journal Volume: | 53 | Journal Issue: | 1 | Start page/Pages: | 487-495 | Source: | Food Research International | Abstract: | Daidzein and genistein have many benefits for human health; however, their applications may be restricted because of their low aqueous solubility. In this work, we generated two water soluble isoflavone derivatives, daidzein 7-O-phosphate and genistein 7-O-phosphate, by incubating Bacillus substilis var. natto BCRC 80517 with daidzein and genistein. These two isoflavone derivatives were characterized by HPLC-ESI-MS/MS, 13C NMR and 31P NMR. Genistein was phosphorylated more rapidly than daidzein. In addition, this bacterial strain could transform glucosidic isoflavones via aglucones into the corresponding 7-O-phosphate conjugates. However, despite the production of agluconic daidzein and genistein, cells could not transform malonyl glucosidic isoflavones into the 7-O-phosphate conjugates. Furthermore, alkaline phosphatase activities from human colon carcinoma (Caco-2) cells were found effective in the dephosphorylation of daidzein 7-O-phosphate and genistein 7-O-phosphate into the corresponding aglyconic isoflavones. ? 2013 Elsevier Ltd. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/446228 | ISSN: | 09639969 | DOI: | 10.1016/j.foodres.2013.05.027 | SDG/Keyword: | Bacillus subtilis natto; Biotransformation; Daidzein; Genistein; Isoflavones; Bacteriology; Nuclear magnetic resonance spectroscopy; Phosphatases; Value engineering; Flavonoids [SDGs]SDG3 |
Appears in Collections: | 農業化學系 |
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