|Title:||Soy isoflavone-phosphate conjugates derived by cultivating Bacillus subtilis var. natto BCRC 80517 with isoflavone||Authors:||Hsu C.
|Issue Date:||2013||Journal Volume:||53||Journal Issue:||1||Start page/Pages:||487-495||Source:||Food Research International||Abstract:||
Daidzein and genistein have many benefits for human health; however, their applications may be restricted because of their low aqueous solubility. In this work, we generated two water soluble isoflavone derivatives, daidzein 7-O-phosphate and genistein 7-O-phosphate, by incubating Bacillus substilis var. natto BCRC 80517 with daidzein and genistein. These two isoflavone derivatives were characterized by HPLC-ESI-MS/MS, 13C NMR and 31P NMR. Genistein was phosphorylated more rapidly than daidzein. In addition, this bacterial strain could transform glucosidic isoflavones via aglucones into the corresponding 7-O-phosphate conjugates. However, despite the production of agluconic daidzein and genistein, cells could not transform malonyl glucosidic isoflavones into the 7-O-phosphate conjugates. Furthermore, alkaline phosphatase activities from human colon carcinoma (Caco-2) cells were found effective in the dephosphorylation of daidzein 7-O-phosphate and genistein 7-O-phosphate into the corresponding aglyconic isoflavones. ? 2013 Elsevier Ltd.
|URI:||https://scholars.lib.ntu.edu.tw/handle/123456789/446228||ISSN:||09639969||DOI:||10.1016/j.foodres.2013.05.027||SDG/Keyword:||Bacillus subtilis natto; Biotransformation; Daidzein; Genistein; Isoflavones; Bacteriology; Nuclear magnetic resonance spectroscopy; Phosphatases; Value engineering; Flavonoids
|Appears in Collections:||農業化學系|
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