Escherichia coli proteome microarrays identified the substrates of clpyq protease
Journal
Molecular and Cellular Proteomics
Journal Volume
16
Journal Issue
1
Pages
113-120
Date Issued
2017
Author(s)
Abstract
between ClpY and YbaB by quartz crystal microbalance. Furthermore, we validated that YbaB was successfully degraded by ClpYQ protease activity using ClpYQ in vitro and in vivo degradation assay. These findings demonstrated the YbaB is a novel substrate of ClpYQ protease. This work also successfully demonstrated that with the use of recognition element of a protease can successfully screen its substrates by indirect proteome chip screening assay.
Type
journal article