Synergistic antiviral effect of Galanthus nivalis agglutinin and nelfinavir against feline coronavirus
Journal
Antiviral Research
Journal Volume
88
Journal Issue
1
Pages
25-30
Date Issued
2010
Abstract
Feline infectious peritonitis (FIP) is a fatal disease in domestic and nondomestic felids caused by feline coronavirus (FCoV). Currently, no effective vaccine is available for the prevention of this disease. In searching for agents that may prove clinically effective against FCoV infection, 16 compounds were screened for their antiviral activity against a local FCoV strain in Felis catus whole fetus-4 cells. The results showed that Galanthus nivalis agglutinin (GNA) and nelfinavir effectively inhibited FCoV replication. When the amount of virus preinoculated into the test cells was increased to mimic the high viral load present in the target cells of FIP cats, GNA and nelfinavir by themselves lost their inhibitory effect. However, when the two agents were added together to FCoV-infected cells, a synergistic antiviral effect defined by complete blockage of viral replication was observed. These results suggest that the combined use of GNA and nelfinavir has therapeutic potential in the prophylaxis and treatment of cats with early-diagnosed FIP. ? 2010 Elsevier B.V.
SDGs
Other Subjects
aciclovir; agglutinin; atazanavir; efavirenz; emodin; idoxuridine; indinavir; lamivudine; lamivudine plus zidovudine; lopinavir plus ritonavir; nelfinavir; nevirapine; promazine; ribavirin; saquinavir; stavudine; animal cell; antiviral activity; article; cell line; cell strain fcwf 4; cell viability; concentration response; controlled study; Coronavirus; drug potentiation; drug screening; feline infectious peritonitis; fetus; Galanthus; galanthus nivalis; IC 50; nonhuman; priority journal; virus inhibition; virus load; virus replication; Animals; Antiviral Agents; Cats; Cells, Cultured; Coronavirus, Feline; Dose-Response Relationship, Drug; Drug Synergism; Drug Therapy, Combination; Feline Infectious Peritonitis; Fetus; Galanthus; Mannose-Binding Lectins; Microbial Sensitivity Tests; Nelfinavir; Plant Lectins; Virus Replication; Felidae; Feline coronavirus; Felis catus; Galanthus nivalis
Type
journal article