|Title:||The detection and differentiation of canine respiratory pathogens using oligonucleotide microarrays||Authors:||Wang L.-C.
|Keywords:||Bordetella bronchiseptica; Canine distemper virus; Canine herpesvirus; Influenza virus; Mycoplasma cynos; Oligonucleotide microarray||Issue Date:||2017||Journal Volume:||243||Start page/Pages:||131-137||Source:||Journal of Virological Methods||Abstract:||
Canine respiratory diseases are commonly seen in dogs along with co-infections with multiple respiratory pathogens, including viruses and bacteria. Virus infections in even vaccinated dogs were also reported. The clinical signs caused by different respiratory etiological agents are similar, which makes differential diagnosis imperative. An oligonucleotide microarray system was developed in this study. The wild type and vaccine strains of canine distemper virus (CDV), influenza virus, canine herpesvirus (CHV), Bordetella bronchiseptica and Mycoplasma cynos were detected and differentiated simultaneously on a microarray chip. The detection limit is 10, 10, 100, 50 and 50 copy numbers for CDV, influenza virus, CHV, B. bronchiseptica and M. cynos, respectively. The clinical test results of nasal swab samples showed that the microarray had remarkably better efficacy than the multiplex PCR-agarose gel method. The positive detection rate of microarray and agarose gel was 59.0% (n?=?33) and 41.1% (n?=?23) among the 56 samples, respectively. CDV vaccine strain and pathogen co-infections were further demonstrated by the microarray but not by the multiplex PCR-agarose gel. The oligonucleotide microarray provides a highly efficient diagnosis alternative that could be applied to clinical usage, greatly assisting in disease therapy and control. ? 2017 Elsevier B.V.
|URI:||https://scholars.lib.ntu.edu.tw/handle/123456789/446433||ISSN:||0166-0934||DOI:||10.1016/j.jviromet.2017.02.004||metadata.dc.subject.other:||agarose; Article; bacterium detection; Bordetella bronchiseptica; Bordetella bronchiseptica infection; Canid herpesvirus 1; canine distemper; Canine distemper virus; controlled study; DNA microarray; dog; herpes virus infection; influenza; Influenza virus; limit of detection; mixed infection; multiplex polymerase chain reaction; Mycoplasma; Mycoplasma cynos; nonhuman; nose smear; respiratory tract infection; virus detection; animal; Bacterial Infections; DNA microarray; Dog Diseases; microarray analysis; molecular diagnosis; procedures; Respiratory Tract Infections; veterinary; Virus Diseases; Animals; Bacterial Infections; Dog Diseases; Dogs; Microarray Analysis; Molecular Diagnostic Techniques; Oligonucleotide Array Sequence Analysis; Respiratory Tract Infections; Virus Diseases
|Appears in Collections:||臨床動物醫學研究所|
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