A convenient nucleic acid test on the basis of the capillary convective PCR for the on-site detection of enterovirus 71
Journal
Journal of Molecular Diagnostics
Journal Volume
16
Journal Issue
4
Pages
452-458
Date Issued
2014
Author(s)
Zhang, S.
Lin, Y.
Wang, J.
Wang, P.
Chen, J.
Xue, M.
He, S.
Zhou, W.
Xu, F.
Liu, P.
Chen, P.
Ge, S.
Xia, N.
Abstract
The recent and continuing epidemic of enterovirus 71 in China has affected millions of children and resulted in thousands of deaths. Timely diagnosis and management is essential for disease control. Current enterovirus 71 molecular tests require resources that are unavailable for on-site testing. We have developed a simple-to-operate nucleic acid test, the convenient and integrated nucleic acid test, for local medical institutions. It uses a convective PCR for rapid amplification, a dipstick for visual detection of PCR products, and a simple commercial kit for nucleic acid extraction. By using a specially designed reagent and reaction tube containing a dipstick, the amplification and detection processes are well integrated and simplified. Moreover, cross contamination that may be caused by an open-tube detection system can be avoided. On the basis of the convenient and integrated nucleic acid test, an enterovirus 71 assay for on-site testing was developed. After evaluating known hand, foot, and mouth disease virus stocks of 17 strains of 11 different serotypes, this assay showed a favorable detection spectrum and no cross-reactivity. Its clinical performance was established by testing 141 clinical samples and comparing the results with a nested RT-PCR method. The assay showed a clinical sensitivity and specificity of 98.5% and 100%, respectively. Our results suggest that this convenient and integrated nucleic acid test enterovirus 71 assay may serve as an on-site diagnosis tool. ? 2014 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.
SDGs
Other Subjects
article; controlled study; cross reaction; diagnostic test; diagnostic test accuracy study; Enterovirus 71; gene amplification; human; human cell; intermethod comparison; nonhuman; nucleic acid analysis; polymerase chain reaction; reverse transcription polymerase chain reaction; sensitivity and specificity; virus detection; child; China; devices; Enterovirus; equipment design; evaluation study; genetics; Hand, Foot and Mouth Disease; isolation and purification; polymerase chain reaction; procedures; virology; virus RNA; Child; China; Enterovirus A, Human; Equipment Design; Hand, Foot and Mouth Disease; Humans; Polymerase Chain Reaction; RNA, Viral; Sensitivity and Specificity
Type
journal article