Nak regulates localization of clathrin sites in higher-order dendrites to promote local dendrite growth
Journal
Neuron
Journal Volume
72
Journal Issue
2
Pages
285-299
Date Issued
2011
Author(s)
Yang W.-K.
Peng Y.-H.
Li H.
Lin H.-C.
Lin Y.-C.
Lai T.-T.
Suo H.
Wang C.-H.
Lin W.-H.
Zhou X.
Pi H.
Chang H.
Chien C.-T.
Abstract
During development, dendrites arborize in a field several hundred folds of their soma size, a process regulated by intrinsic transcription program and cell adhesion molecule (CAM)-mediated interaction. However, underlying cellular machineries that govern distal higher-order dendrite extension remain largely unknown. Here, we show that Nak, a clathrin adaptor-associated kinase, promotes higher-order dendrite growth through endocytosis. In nak mutants, both the number and length of higher-order dendrites are reduced, which are phenocopied by disruptions of clathrin-mediated endocytosis. Nak interacts genetically with components of the endocytic pathway, colocalizes with clathrin puncta, and is required for dendritic localization of clathrin puncta. More importantly, these Nak-containing clathrin structures preferentially localize to branching points and dendritic tips that are undergoing active growth. We present evidence that the Drosophila L1-CAM homolog Neuroglian is a relevant cargo of Nak-dependent internalization, suggesting that localized clathrin-mediated endocytosis of CAMs facilitates the extension of nearby higher-order dendrites.
SDGs
Type
journal article