Cell therapy for salivary gland regeneration
Journal
Journal of Dental Research
Journal Volume
90
Journal Issue
3
Pages
341-346
Date Issued
2011
Author(s)
Abstract
There are still no effective therapies for hyposalivation caused by irradiation. In our previous study, bone marrow stem cells can be transdifferentiated into acinar-like cells in vitro. Therefore, we hypothesized that transplantation with bone marrow stem cells or acinar-like cells may help functional regeneration of salivary glands. Bone marrow stem cells were labeled with nanoparticles and directly co-cultured with acinar cells to obtain labeled acinar-like cells. In total, 140 severely combined immune-deficiency mice were divided into 4 groups for cell therapy experiments: (1) normal mice, (2) mice receiving irradiation around their head-and-neck areas; (3) mice receiving irradiation and intra-gland transplantation with labeled stem cells; and (4) mice receiving irradiation and intra-gland transplantation with labeled acinar-like cells. Our results showed that salivary glands damaged due to irradiation can be rescued by cell therapy with either bone marrow stem cells or acinar-like cells for recovery of saliva production, body weight, and gland weight. Transdifferentiation of bone marrow stem cells into acinar-like cells in vivo was also noted. This study demonstrated that cell therapy with bone marrow stem cells or acinar-like cells can help functional regeneration of salivary glands, and that acinar-like cells showed better therapeutic potentials than those of bone marrow stem cells. ? International & American Associations for Dental Research.
SDGs
Other Subjects
amylase; ferric ion; ferric oxide; magnetite nanoparticle; animal; article; biological therapy; biosynthesis; bone marrow transplantation; coculture; cytology; epithelium cell; mesenchymal stem cell transplantation; methodology; mouse; mouse mutant; nonobese diabetic mouse; radiation exposure; radiation injury; regeneration; reverse transcription polymerase chain reaction; salivary gland; skull irradiation; transplantation; xerostomia; Amylases; Animals; Bone Marrow Transplantation; Coculture Techniques; Cranial Irradiation; Epithelial Cells; Ferric Compounds; Magnetite Nanoparticles; Mesenchymal Stem Cell Transplantation; Mice; Mice, Inbred NOD; Mice, SCID; Radiation Injuries, Experimental; Regeneration; Reverse Transcriptase Polymerase Chain Reaction; Salivary Glands; Tissue Therapy; Xerostomia
Type
journal article