Cell therapy for salivary gland regeneration
Journal
Journal of Dental Research
Journal Volume
90
Journal Volume
90
Journal Issue
3
Journal Issue
3
Pages
341-346
Start Page
341
End Page
346
Date Issued
2011
Author(s)
Abstract
There are still no effective therapies for hyposalivation caused by irradiation. In our previous study, bone marrow stem cells can be transdifferentiated into acinar-like cells in vitro. Therefore, we hypothesized that transplantation with bone marrow stem cells or acinar-like cells may help functional regeneration of salivary glands. Bone marrow stem cells were labeled with nanoparticles and directly co-cultured with acinar cells to obtain labeled acinar-like cells. In total, 140 severely combined immune-deficiency mice were divided into 4 groups for cell therapy experiments: (1) normal mice, (2) mice receiving irradiation around their head-and-neck areas; (3) mice receiving irradiation and intra-gland transplantation with labeled stem cells; and (4) mice receiving irradiation and intra-gland transplantation with labeled acinar-like cells. Our results showed that salivary glands damaged due to irradiation can be rescued by cell therapy with either bone marrow stem cells or acinar-like cells for recovery of saliva production, body weight, and gland weight. Transdifferentiation of bone marrow stem cells into acinar-like cells in vivo was also noted. This study demonstrated that cell therapy with bone marrow stem cells or acinar-like cells can help functional regeneration of salivary glands, and that acinar-like cells showed better therapeutic potentials than those of bone marrow stem cells. ? International & American Associations for Dental Research.
SDGs
Other Subjects
amylase; ferric ion; ferric oxide; magnetite nanoparticle; animal; article; biological therapy; biosynthesis; bone marrow transplantation; coculture; cytology; epithelium cell; mesenchymal stem cell transplantation; methodology; mouse; mouse mutant; nonobese diabetic mouse; radiation exposure; radiation injury; regeneration; reverse transcription polymerase chain reaction; salivary gland; skull irradiation; transplantation; xerostomia; Amylases; Animals; Bone Marrow Transplantation; Coculture Techniques; Cranial Irradiation; Epithelial Cells; Ferric Compounds; Magnetite Nanoparticles; Mesenchymal Stem Cell Transplantation; Mice; Mice, Inbred NOD; Mice, SCID; Radiation Injuries, Experimental; Regeneration; Reverse Transcriptase Polymerase Chain Reaction; Salivary Glands; Tissue Therapy; Xerostomia
Type
journal article