Monoclonal antibody-functionalized mesoporous silica nanoparticles (MSN) for selective targeting breast cancer cells
Journal
Journal of Materials Chemistry
Journal Volume
19
Journal Issue
32
Pages
5737-5743
Date Issued
2009
Author(s)
Abstract
In this work, we conjugate anti-HER2/neu mAb (monoclonal antibody) to green fluorescent dye loaded mesoporous silica nanoparticles (Her-Dye@MSN) through a polyethylene glycol (MW = 5000) spacer. We examine their targeting properties toward HER2/neu over-expressing breast cancer cells and their internalization into the cells. Her-Dye@MSN nanoparticles exhibit a high targeting efficiency and the specific targeting capability is strongly affected by the mAb density on the Her-Dye@MSN. The time-course of competitive experiments with free antibody indicates that Her-Dye@MSN serves as a multivalent ligand. Moreover, Her-Dye@MSNs are internalized into cells through a receptor-mediated endocytosis and may escape from endosome to cytosol. The results reported here further support the potential of MSNs as a multifunctional smart nanocarriers for cell imaging and drug delivery. ? 2009 The Royal Society of Chemistry.
SDGs
Other Subjects
Breast cancer cells; Cell imaging; Competitive experiments; Cytosols; Endosomes; Fluorescent dyes; Functionalized; Mesoporous silica nanoparticles; Multivalent ligands; Nanocarriers; Receptor-mediated endocytosis; Targeting capability; Drug delivery; Mesoporous materials; Monoclonal antibodies; Nanoparticles; Polyethylene glycols; Polyethylene oxides; Silica; Surface plasmon resonance; Cell membranes
Type
journal article