Identification of complement C3a as a candidate biomarker in human chronic hepatitis C and HCV-related hepatocellular carcinoma using a proteomics approach
Journal
Proteomics
Journal Volume
6
Journal Issue
9
Pages
2865-2873
Date Issued
2006
Author(s)
Lee I.-N.
Yu C.-Y.
Wen C.-L.
Lu F.-J.
Abstract
Although the significant risk factors for hepatocellular carcinoma (HCC) are well known from epidemiological studies, diagnosis of this disease at an early stage is difficult, and HCC remains one of the leading causes of cancer death worldwide. Thus, to identify any useful HCC-related biomarkers is still a need. We performed SELDI-TOF MS to identify differentially expressed proteins in HCC serum using weak cation exchange protein chips. Protein characterization was performed by 2-DE separation and nano flow LC-MS/MS. A total of 55 sera were collected from HCC patients and compared with those from 48 patients with chronic hepatitis and 9 healthy individuals. A candidate marker of about 8900 Da was detected as differentially expressed in patients with chronic hepatitis C and hepatitis C virus (HCV)-related HCC. We identified this differentially expressed protein as complement C3a. The expression of C3a in HCC sera was further validated by PS20 chip immunoassay and Western blotting. Complement C3a was found to be elevated in patients with chronic hepatitis C and HCV-related HCC. The combination of SELDI-TOF MS and 2-DE provides a solution to identify disease-associated serum biomarkers. ? 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
SDGs
Other Subjects
biological marker; complement component C3a; plasma protein; adult; aged; article; cation exchange; controlled study; hepatitis C; Hepatitis C virus; human; immunoassay; liquid chromatography; liver cell carcinoma; major clinical study; mass spectrometry; priority journal; protein analysis; protein expression; protein microarray; proteomics; separation technique; surface enhanced laser desorption ionization time of flight mass spectrometry; Western blotting; Aged; Blotting, Western; Carcinoma, Hepatocellular; Complement C3a; Electrophoresis, Gel, Two-Dimensional; Hepatitis C, Chronic; Humans; Middle Aged; Protein Array Analysis; Proteomics; Reference Values; Reproducibility of Results; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tumor Markers, Biological; Hepatitis C virus
Type
journal article