https://scholars.lib.ntu.edu.tw/handle/123456789/452443
標題: | Sub-proteome analysis of novel IgE-binding proteins from Bermuda grass pollen | 作者: | Kao S.-H. Su S.-N. Huang S.-W. Tsai J.-J. LU-PING CHOW |
公開日期: | 2005 | 卷: | 5 | 期: | 14 | 起(迄)頁: | 3805-3813 | 來源出版物: | Proteomics | 摘要: | Bermuda grass (Cynodon dactylon) pollen (BGP) is one of the most common causes of airway allergic disease, and has been shown to contain over 12 allergenic proteins on 1-D immunoglobulin E (IgE) immunoblots. However, only a few allergens have been identified and characterized. Cyn d 1 is a major allergen and the most abundant protein in BGP, representing 15% of the whole-pollen extract. To investigate variability in the IgE-reactive patterns of BGP-sensitized patients and to identify other prevalent allergens, a BGP extract was passed through an affinity column to remove Cyn d 1, and the non-bound material was collected and analyzed by 2-DE. IgE-reactive proteins were subsequently characterized by immunoblotting using serum samples from ten BGP-allergic patients. The prevalent IgE-reactive proteins were identified by MALDI-TOF MS, N-terminal sequence similarity, and LC-MS/MS. Here, we present a sub-proteome approach for allergen investigation and its use for determining BGP 2-DE profiles and identifying six novel allergens. ? 2005 Wiley-VCH Verlag GmbH & Co. KGaA. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-25844477996&doi=10.1002%2fpmic.200401229&partnerID=40&md5=58a1aed6c30bee0a7cebb629388c44f9 https://scholars.lib.ntu.edu.tw/handle/123456789/452443 |
ISSN: | 16159853 | DOI: | 10.1002/pmic.200401229 | SDG/關鍵字: | allergen; binding protein; immunoglobulin E; proteome; article; binding affinity; controlled study; grass pollen; human; immunoblotting; liquid chromatography; matrix assisted laser desorption ionization time of flight mass spectrometry; priority journal; protein analysis; proteomics; tandem mass spectrometry; two dimensional electrophoresis; Blotting, Western; Cynodon; Immunoglobulin E; Plant Proteins; Proteome; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Cynodon dactylon |
顯示於: | 生物化學暨分子生物學科研究所 |
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