Dietary flavonoids luteolin and quercetin suppressed cancer stem cell properties and metastatic potential of isolated prostate cancer cells
Journal
Anticancer Research
Journal Volume
36
Journal Issue
12
Pages
6367 - 6380
Date Issued
2016
Author(s)
Tsai, Pei-Hsun
Cheng, Chia-Hsiung
Lin, Chun-Yu
Huang, Ying-Tang
Lee, Lung-Ta
Kandaswami, Chithan C.
Lin, Yo-Chuen
Lee, Kevin Po-Hao
Hung, Chin-Chun
Hwang, Jiuan-Jiuan
Ke, Ferng-Chun
Lee, Ming-Ting
Abstract
A highly invasive Du145-III subline was isolated by three successive passages of the parental Du145 prostate tumor cell line (Du145-P) through a Boyden chamber with matrigel-coated membrane support. Du145-III cells showed great invasion potential based on their increased ability to spread/migrate and enhanced expression/secretion of the matrix metalloproteinase 9 (MMP9). Du145-III cells exerted vasculogenic mimicry (VM) properties, reminiscent of endothelial cell characteristics and expressed elevated levels of cancer stem cell (CSC) markers, including Nanog, Sox2, CD44 and ABCG2 and ability to self-renew. Of prominence, MMP9 was required for the induction of VM and for increased stemness in Du145-III cells. Using Du145-III as a model, the effects of dietary flavonoids, luteolin and quercetin, were evaluated on stemness and invasion capacity of Du145-III cells in relation to JNK signaling pathway activation. These flavonoids depressed the malignancy of highly invasive Du145-III cells, VM, anchorage-independent spheroid formation and expression of certain CSC markers. Since luteolin and quercetin were able to target CSC cells and prevent cancer cell invasiveness, may serve as potential anti-angiogenesis and anti-metastasis agents.
Subjects
Cancer stem cell; Luteolin; Metastasis; Prostate cancer; Quercetin; Vasculogenic mimicry
SDGs
Other Subjects
breast cancer resistance protein; gelatinase B; Hermes antigen; luteolin; quercetin; transcription factor NANOG; transcription factor Sox2; luteolin; quercetin; Article; cancer stem cell; controlled study; drug effect; enzyme release; metastasis potential; priority journal; prostate cancer; prostate cancer cell line; protein expression; stem cell self-renewal; cancer stem cell; chemistry; diet; drug effects; human; male; pathology; prostate tumor; Diet; Humans; Luteolin; Male; Neoplastic Stem Cells; Prostatic Neoplasms; Quercetin
Type
journal article