|Title:||Methylated ZNF582 gene as a marker for triage of women with Pap smear reporting low-grade squamous intraepithelial lesions - A Taiwanese Gynecologic Oncology Group (TGOG) study||Authors:||Lin H.
|Issue Date:||2014||Journal Volume:||135||Journal Issue:||1||Start page/Pages:||64-68||Source:||Gynecologic Oncology||Abstract:||
Objective. Our previous work revealed that host genes ZNF582, PTPRR, PAX1, and SOX1 are highlymethylated in cervical intraepithelial neoplasias grade 3 orworse (CIN3+). In this study,we used a standardized testing assay to evaluate the clinical efficacy of these biomarkers in the triage of cytological diagnoses of low-grade squamous intraepithelial lesions (LSILs), and compared the performance with human papillomavirus (HPV) testing.Methods. This 2-year multicenter prospective study examined a population of 230 women from 12 medical centers who were diagnosed with LSILs on cervical cytology. Cervical scrapings were obtained prior to a colposcopy-directed biopsy for quantitative methylation analysis of ZNF582, PTPRR, PAX1, and SOX1, and HPV testing. Using logistic regression and receiver operating characteristic curve analyses, the abilities of methylated genes and HPV to predict CIN3+ were assessed.Results. Fifteen (6.5%) of the 230 women with a cytological diagnosis of LSIL were confirmed to have CIN3+ after a colposcopy-directed biopsy. Among the 4 methylated genes, ZNF582 was found to be the best biomarker for detecting CIN3+. The sensitivities for methylated ZNF582 and HPV testing were 73% and 80%, and the specificities were 71% and 28%, respectively. The odds ratio for predicting CIN3+ using methylated ZNF582 was 6.8 (95% confidence interval (CI) 2.1-22.1), which was much better than HPV testing (OR = 1.6, 95% CI 0.4-5.8).Conclusion. This is the first study to show that ZNF582 methylation analysis of cervical swabs may be a promising choice in the positive triage of cytological diagnoses of LSILs. ? 2014 Elsevier Inc.
|URI:||https://scholars.lib.ntu.edu.tw/handle/123456789/452909||DOI:||10.1016/j.ygyno.2014.08.012||SDG/Keyword:||biological marker; genetic marker; kruppel like factor; ZNF582 protein, human; adult; Article; cancer cytodiagnosis; cancer grading; colposcopy; controlled study; diagnostic test accuracy study; DNA methylation; female; human; human tissue; major clinical study; marker gene; multicenter study; Papanicolaou test; PAX1 gene; PTPRR gene; sensitivity and specificity; SOX1 gene; Taiwan; uterine cervix biopsy; uterine cervix carcinoma in situ; uterine cervix cytology; Wart virus; ZNF582 gene; DNA methylation; genetic marker; genetics; isolation and purification; metabolism; middle aged; Papanicolaou test; Papilloma virus; pathology; prospective study; squamous intraepithelial lesion of the cervix; uterine cervix carcinoma in situ; vagina smear; virology; Adult; Cervical Intraepithelial Neoplasia; DNA Methylation; Female; Genetic Markers; Humans; Kruppel-Like Transcription Factors; Middle Aged; Neoplasm Grading; Papanicolaou Test; Papillomaviridae; Prospective Studies; Squamous Intraepithelial Lesions of the Cervix; Vaginal Smears
|Appears in Collections:||醫學系|
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