https://scholars.lib.ntu.edu.tw/handle/123456789/454619
標題: | Arctigenin from Arctium lappa inhibits interleukin-2 and interferon gene expression in primary human T lymphocytes | 作者: | Tsai W.-J. Chang C.-T. Wang G.-J. Lee T.-H. Chang S.-F. SHAO-CHUN LU Kuo Y.-C. |
公開日期: | 2011 | 卷: | 6 | 來源出版物: | Chinese Medicine | 摘要: | Background: Arctium lappa (Niubang), a Chinese herbal medicine, is used to treat tissue inflammation. This study investigates the effects of arctigenin (AC), isolated from A. lappa, on anti-CD3/CD28 Ab-stimulated cell proliferation and cytokine gene expression in primary human T lymphocytes.Methods: Cell proliferation was determined with enzyme immunoassays and the tritiated thymidine uptake method. Cytokine production and gene expression were analyzed with reverse transcription-polymerase chain reaction.Results: AC inhibited primary human T lymphocytes proliferation activated by anti-CD3/CD28 Ab. Cell viability test indicated that the inhibitory effects of AC on primary human T lymphocyte proliferation were not due to direct cytotoxicity. AC suppressed interleukin-2 (IL-2) and interferon-γ (IFN-γ) production in a concentration-dependent manner. Furthermore, AC decreased the IL-2 and IFN-γ gene expression in primary human T lymphocytes induced by anti-CD3/CD28 Ab. Reporter gene analyses revealed that AC decreased NF-AT-mediated reporter gene expression.Conclusion: AC inhibited T lymphocyte proliferation and decreased the gene expression of IL-2, IFN-γ and NF-AT. ? 2011 Tsai et al; licensee BioMed Central Ltd. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-79953092808&doi=10.1186%2f1749-8546-6-12&partnerID=40&md5=c5b449477d63b8320c0f5323ccecdb03 https://scholars.lib.ntu.edu.tw/handle/123456789/454619 |
ISSN: | 1749-8546 | DOI: | 10.1186/1749-8546-6-12 | SDG/關鍵字: | arctigenin; CD3 antibody; gamma interferon; glyceraldehyde 3 phosphate dehydrogenase (NADP); interleukin 2; monoclonal antibody CD28; transcription factor NFAT; adult; Arctium; article; cell viability; controlled study; cytokine production; cytotoxicity; drug effect; gene expression; human; human cell; interferon production; lymphocyte proliferation; male; normal human; reporter gene; T lymphocyte |
顯示於: | 生物化學暨分子生物學科研究所 |
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