https://scholars.lib.ntu.edu.tw/handle/123456789/454735
標題: | TMPRSS2, a serine protease expressed in the prostate on the apical surface of luminal epithelial cells and released into semen in prostasomes, is misregulated in prostate cancer cells | 作者: | Chen Y.-W. MING-SHYUE LEE Lucht A. Chou F.-P. Huang W. Havighurst T.C. Kim K. Wang J.-K. Antalis T.M. Johnson M.D. Lin C.-Y. |
公開日期: | 2010 | 出版社: | American Society for Investigative Pathology Inc. | 卷: | 176 | 期: | 6 | 起(迄)頁: | 2986-2996 | 來源出版物: | American Journal of Pathology | 摘要: | TMPRSS2, a type II transmembrane serine protease, is highly expressed by the epithelium of the human prostate gland. To explore the regulation and function of TMPRSS2 in the prostate, a panel of monoclonal antibodies with high sensitivity and specificity were generated. Immunodetection showed TMPRSS2 on the apical plasma membrane of the prostate luminal cells and demonstrated its release into semen as a component of prostasomes, organelle-like vesicles that may facilitate sperm function and enhance male reproduction. In prostate cancer cells, TMPRSS2 expression was increased and the protein mislocalized over the entire tumor cell membrane. In both LNCaP prostate cancer cells and human semen, TMPRSS2 protein was detected predominantly as inactive zymogen forms as part of an array of multiple noncovalent and disulfide-linked complexes, suggesting that TMPRSS2 activity may be regulated by unconventional mechanisms. Our data suggested that TMPRSS2, an apical surface serine protease, may have a normal role in male reproduction as a component of prostasomes. The aberrant cellular localization, and increased expression of the protease seen in cancer, may contribute to prostate tumorigenesis by providing access of the enzyme to nonphysiological substrates and binding-proteins. Copyright ? American Society for Investigative Pathology. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-77953196179&doi=10.2353%2fajpath.2010.090665&partnerID=40&md5=23b7c0bed512880e6f3467abb3bd5428 https://scholars.lib.ntu.edu.tw/handle/123456789/454735 |
ISSN: | 0002-9440 | DOI: | 10.2353/ajpath.2010.090665 | SDG/關鍵字: | serine proteinase; tmprss2; unclassified drug; monoclonal antibody; serine proteinase; TMPRSS2 protein, human; article; cancer cell; carcinogenesis; cell membrane; cellular distribution; controlled study; epithelium cell; human; human cell; immunodetection; male; male genital system; priority journal; prostate cancer; protein analysis; protein expression; sensitivity and specificity; sperm; adult; aged; animal; cytology; enzymology; epithelium cell; genetics; metabolism; microarray analysis; middle aged; mouse; pathology; prostate; prostate tumor; sperm; tumor cell line; very elderly; Adult; Aged; Aged, 80 and over; Animals; Antibodies, Monoclonal; Cell Line, Tumor; Epithelial Cells; Humans; Male; Mice; Microarray Analysis; Middle Aged; Prostate; Prostatic Neoplasms; Semen; Serine Endopeptidases |
顯示於: | 生物化學暨分子生物學科研究所 |
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