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  1. NTU Scholars
  2. 醫學院
  3. 醫學系
Please use this identifier to cite or link to this item: https://scholars.lib.ntu.edu.tw/handle/123456789/455316
Title: 3D porous calcium-alginate scaffolds cell culture system improved human osteoblast cell clusters for cell therapy
Authors: Chen, C.-Y.
Ke, C.-J.
Yen, K.-C.
Hsieh, H.-C.
JUI-SHENG SUN 
FENG-HUEI LIN 
Issue Date: 2015
Publisher: Ivyspring International Publisher
Journal Volume: 5
Journal Issue: 6
Start page/Pages: 643-655
Source: Theranostics
Abstract: 
Age-related orthopedic disorders and bone defects have become a critical public health issue, and cell-based therapy is potentially a novel solution for issues surrounding bone tissue engineering and regenerative medicine. Long-term cultures of primary bone cells exhibit phenotypic and functional degeneration; therefore, culturing cells or tissues suitable for clinical use remain a challenge. A platform consisting of human osteoblasts (hOBs), calcium-alginate (Ca-Alginate) scaffolds, and a self-made bioreactor system was established for autologous transplantation of human osteoblast cell clusters. The Ca-Alginate scaffold facilitated the growth and differentiation of human bone cell clusters, and the functionally-closed process bioreactor system supplied the soluble nutrients and osteogenic signals required to maintain the cell viability. This system preserved the proliferative ability of cells and cell viability and up-regulated bone-related gene expression and biological apatite crystals formation. The bone-like tissue generated could be extracted by removal of calcium ions via ethylenediaminetetraacetic acid (EDTA) chelation, and exhibited a size suitable for injection. The described strategy could be used in therapeutic application and opens new avenues for surgical interventions to correct skeletal defects. ? 2015 Ivyspring International Publisher.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84930622801&doi=10.7150%2fthno.11372&partnerID=40&md5=bf3f40770c89023ef36294a4a41206cc
https://scholars.lib.ntu.edu.tw/handle/123456789/455316
ISSN: 1838-7640
DOI: 10.7150/thno.11372
SDG/Keyword: alkaline phosphatase; beta 2 microglobulin; calcium alginate; collagen type 1; edetic acid; messenger RNA; osteocalcin; transcription factor RUNX2; alginic acid; glucuronic acid; hexuronic acid; Alp1 gene; Article; autotransplantation; B2M gene; Bglap gene; bioreactor; bone density; bone development; bone mass; cell proliferation; cell transplantation; cell viability; chelation; Col1a1 gene; controlled study; crystallization; culture technique; gene expression regulation; human; human cell; osteoblast; porosity; process development; Runx2 gene; scanning electron microscopy; three dimensional cell culture system; three dimensional imaging; tissue engineering; tissue scaffold; cell culture; chemistry; cytology; devices; drug effects; osteoblast; physiology; procedures; tissue scaffold; Alginates; Bioreactors; Cell Proliferation; Cells, Cultured; Glucuronic Acid; Hexuronic Acids; Humans; Osteoblasts; Tissue Engineering; Tissue Scaffolds
[SDGs]SDG3
Appears in Collections:醫學系

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臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

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