https://scholars.lib.ntu.edu.tw/handle/123456789/455872
標題: | Core/shell multicellular spheroids on chitosan as in vitro 3D coculture tumor models | 作者: | Tsai C.-W. JYH-HORNG WANG Tai-Horng Young |
公開日期: | 2018 | 出版社: | Taylor and Francis Ltd. | 卷: | 46 | 期: | sup3 | 起(迄)頁: | S651-S660 | 來源出版物: | Artificial Cells, Nanomedicine and Biotechnology | 摘要: | An ideal in vitro drug screening model is important for the drug development. In addition to monoculture systems, 3 dimensional (3D) coculture systems are extensively used to simulate the in vivo tumor microenvironment as cell-cell and cell-extracellular matrix interactions within the tumor tissues can be mimicked. In this study, in vitro 3D suspension coculture multicellular spheroids with core/shell cell distribution were developed on chitosan-coated surfaces. Based on the characteristic of chitosan inhibiting cell adhesion, SW620 (colon cancer cell line), 3A6 (mesenchymal stem-like cell line) and Hs68 (foreskin fibroblast line) cells could aggregate to form 3D coculture spheroids with intimate cell contacts. When cells were cocultured on chitosan, 3A6 and Hs68 cells always located in the core of spheroids and were completely enveloped by SW620 cells due to their N-cadherin protein expression following the differential adhesion hypothesis. The core cells could be the feeder layers to stimulate the shell SW620 cells to enhance their mitochondria activity. Moreover, 3D coculture core/shell multicellular spheroids could enhance the resistance of SW620 cells against the cytotoxicity effect of chemotherapy drugs. To sum up, based on the specificity of the core/shell coculture multicellular spheroids, a novel in vitro tumor model was proposed in this study. ? 2018, ? 2018 Informa UK Limited, trading as Taylor & Francis Group. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85054916459&doi=10.1080%2f21691401.2018.1505744&partnerID=40&md5=641967299aeef06053401ae0884a9884 https://scholars.lib.ntu.edu.tw/handle/123456789/455872 |
ISSN: | 2169-1401 | DOI: | 10.1080/21691401.2018.1505744 | SDG/關鍵字: | Cell adhesion; Cell culture; Chemotherapy; Chitosan; Diagnosis; Enzyme activity; Glycoproteins; Self assembly; Tumors; 3-d cultures; Colon cancer cells; Cytotoxicity effects; Extracellular matrix interactions; Multicellular spheroid; N-cadherin; Tumor microenvironment; Tumor models; Cells; chitosan; doxorubicin; nerve cell adhesion molecule; P cadherin; uvomorulin; chitosan; Article; cell adhesion; cell contact; cellular distribution; coculture; controlled study; cytotoxicity; drug screening; extracellular matrix; Hs 683 cell line; human; human cell; in vitro study; multicellular spheroid; protein expression; SW620 cell line; tumor microenvironment; tumor model; tumor spheroid; biological model; chemistry; coculture; colon tumor; fibroblast; mesenchymal stem cell; metabolism; multicellular spheroid; pathology; tumor cell line; Cell Line, Tumor; Chitosan; Coculture Techniques; Colonic Neoplasms; Fibroblasts; Humans; Mesenchymal Stem Cells; Models, Biological; Spheroids, Cellular |
顯示於: | 醫學系 |
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