Divergent endometrial inflammatory cytokine expression at peri-implantation period and after the stimulation by copper intrauterine device
Journal
Scientific Reports
Journal Volume
5
Pages
15157
Date Issued
2015
Author(s)
Abstract
Endometrial inflammation has contradictory effects. The one occurring at peri-implantation period is favourable for embryo implantation, whereas the other occurring after the stimulation by copper intrauterine device (Cu-IUD) prevents from embryo implantation. In this study, 8 week female ICR mice were used to investigate the endometrial inflammation, in which they were at proestrus stage (Group 1), at peri-implantation period (Group 2), and had a copper wire implanted into right uterine horn (Group 3). Cytokine array revealed that two cytokines were highly expressed in Group 2 and Group 3 as compared with Group 1, and seven cytokines, including tumour necrosis factor α (TNF-α), had selectively strong expression in Group 3. Immunohistochemistry demonstrated prominent TNF-α staining on the endometrium after Cu-IUD stimulation, and in vitro culture of human endometrial glandular cells with Cu induced TNF-α secretion. The increased TNF-α concentration enhanced in vitro THP-1 cells chemotaxis, and reduced embryo implantation rates. These results suggest that inflammatory cytokine profiles of endometrium are different between those at peri-implantation period and after Cu-IUD stimulation, and TNF-α is the one with selectively strong expression in the latter. It might account for the contradictory biological effects of endometrial inflammation.
SDGs
Other Subjects
copper; cytokine; tumor necrosis factor; animal; cell culture; chemistry; chemotaxis; copper intrauterine device; cytology; drug effects; endometrium; female; human; immunohistochemistry; inflammation; Institute for Cancer Research mouse; metabolism; mouse; nidation; protein microarray; secretion (process); Animals; Cells, Cultured; Chemotaxis; Copper; Cytokines; Embryo Implantation; Endometrium; Female; Humans; Immunohistochemistry; Inflammation; Intrauterine Devices, Copper; Mice; Mice, Inbred ICR; Protein Array Analysis; Tumor Necrosis Factor-alpha
Type
journal article