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  1. NTU Scholars
  2. 醫學院
  3. 醫學系
Please use this identifier to cite or link to this item: https://scholars.lib.ntu.edu.tw/handle/123456789/458616
Title: Clinical significance of signal pattern of high-risk human papillomavirus using a novel fluorescence in situ hybridization assay in cervical cytology
Authors: Ho C.-M.
Lee B.-H.
Chang S.-F.
Chien T.-Y.
Huang S.-H.
Yan C.-C.
WEN-FANG CHENG 
Issue Date: 2011
Journal Volume: 17
Journal Issue: 3
Start page/Pages: 386-394
Source: Clinical Microbiology and Infection
Abstract: 
The present study aimed to evaluate a novel fluorescence in situ hybridization (FISH) assay for detecting the high-risk human papillomavirus (HR-HPV) DNA and signal pattern in cervical cytology specimens and for identifying cervical intraepithelial neoplasia (CIN) lesions. One hundred and ninety-six liquid-based cytology specimens with CIN were recruited. The signal pattern (punctate, mixed punctate and diffuse, and diffuse) detected by FISH was compared with E6 mRNA and correlated with histological classification. FISH and E6-type specific polymerase chain reaction (PCR) had fair to good agreement for detecting HPV DNA across all grades of CIN (kappa coefficient, 0.37-0.73). Among 44 samples of negative FISH and positive E6 type-specific PCR in HPV 16, 18, 31, 33, 52 and 58, 82% (36/44) of E6 mRNA were not detected, in contrast to 41% (48/118) of positive FISH and positive E6 type-specific PCR (p<0.0001). Among HR-HPV DNA positive cases tested by the FISH assay, the specificity of predicting CIN3 using the punctuate pattern is higher than that using E6 mRNA (96.3% vs. 44.8%). The punctate pattern was 0% in patients with
URI: 2-s2.0-79751480818
https://scholars.lib.ntu.edu.tw/handle/123456789/458616
ISSN: 1198-743X
DOI: 10.1111/j.1469-0691.2010.03186.x
SDG/Keyword: messenger RNA; virus DNA; article; clinical assessment; colposcopy; controlled study; diagnostic test accuracy study; DNA extraction; female; fluorescence in situ hybridization; genotype; HeLa cell; human; human tissue; major clinical study; polymerase chain reaction; priority journal; risk assessment; sensitivity and specificity; uterine cervix carcinoma in situ; uterine cervix cytology; virus detection; Wart virus; Human papillomavirus; Human papillomavirus type 16
[SDGs]SDG3
Appears in Collections:醫學系

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臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

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開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

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