Clinical significance of signal pattern of high-risk human papillomavirus using a novel fluorescence in situ hybridization assay in cervical cytology
Journal
Clinical Microbiology and Infection
Journal Volume
17
Journal Issue
3
Pages
386-394
Date Issued
2011
Author(s)
Abstract
The present study aimed to evaluate a novel fluorescence in situ hybridization (FISH) assay for detecting the high-risk human papillomavirus (HR-HPV) DNA and signal pattern in cervical cytology specimens and for identifying cervical intraepithelial neoplasia (CIN) lesions. One hundred and ninety-six liquid-based cytology specimens with CIN were recruited. The signal pattern (punctate, mixed punctate and diffuse, and diffuse) detected by FISH was compared with E6 mRNA and correlated with histological classification. FISH and E6-type specific polymerase chain reaction (PCR) had fair to good agreement for detecting HPV DNA across all grades of CIN (kappa coefficient, 0.37-0.73). Among 44 samples of negative FISH and positive E6 type-specific PCR in HPV 16, 18, 31, 33, 52 and 58, 82% (36/44) of E6 mRNA were not detected, in contrast to 41% (48/118) of positive FISH and positive E6 type-specific PCR (p<0.0001). Among HR-HPV DNA positive cases tested by the FISH assay, the specificity of predicting CIN3 using the punctuate pattern is higher than that using E6 mRNA (96.3% vs. 44.8%). The punctate pattern was 0% in patients with
SDGs
Other Subjects
messenger RNA; virus DNA; article; clinical assessment; colposcopy; controlled study; diagnostic test accuracy study; DNA extraction; female; fluorescence in situ hybridization; genotype; HeLa cell; human; human tissue; major clinical study; polymerase chain reaction; priority journal; risk assessment; sensitivity and specificity; uterine cervix carcinoma in situ; uterine cervix cytology; virus detection; Wart virus; Human papillomavirus; Human papillomavirus type 16
Type
journal article