Type-specific human papillomavirus oncogene messenger RNA levels correlate with the severity of cervical neoplasia
Journal
International Journal of Cancer
Journal Volume
127
Journal Issue
3
Pages
622-632
Date Issued
2010
Author(s)
Abstract
This study aimed to evaluate whether quantitation of high-risk human papillomavirus (HR-HPV) E6 messenger RNA (mRNA) can be a potential biomarker for detecting the severity of cervical lesions. HPV genotyping was performed using a modified MY11/ GP6+ PCR for HPV DNA amplification, followed by HPV genotype-specific hybridization with on a gene chip. E6 type-specific PCR was used to validate multiple infections. Quantitative real-time reverse transcriptase (QRT-PCR) and real-time PCR used to measure mRNA levels and DNA viral loads of 6 HPV oncogenic types (HPV 16, 18, 31, 33, 52 and 58) in 720 liquid-based cytology samples. The HPV DNA and RNA measurements were correlated with cervical lesions diagnosed by histopathologic examination. mRNA transcripts in the 6 types HPV DNA-positive cases was lower in normal women and
SDGs
Other Subjects
messenger RNA; protein E6; tumor marker; virus DNA; virus RNA; adult; article; controlled study; cross-sectional study; cytology; disease severity; DNA hybridization; female; gene amplification; genotype; histopathology; human; Human papillomavirus type 16; Human papillomavirus type 18; Human papillomavirus type 31; Human papillomavirus type 33; Human papillomavirus type 52; Human papillomavirus type 58; major clinical study; mixed infection; nucleotide sequence; priority journal; quantitative analysis; real time polymerase chain reaction; RNA transcription; uterine cervix carcinoma in situ; validation process; virus load; virus oncogene; Wart virus; Alphapapillomavirus; Base Sequence; Cross-Sectional Studies; DNA Primers; Female; Humans; Nucleic Acid Hybridization; Oncogenes; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Severity of Illness Index; Uterine Cervical Neoplasms; Viral Load
Type
journal article