https://scholars.lib.ntu.edu.tw/handle/123456789/458631
Title: | Noncarrier naked antigen-specific DNA vaccine generates potent antigen-specific immunologic responses and antitumor effects | Authors: | CHI-AN CHEN Chang M.-C. WEI-ZEN SUN YU-LI CHEN YING-CHENG CHIANG CHANG-YAO HSIEH Chen S.M. Hsiao P.-N. WEN-FANG CHENG |
Issue Date: | 2009 | Journal Volume: | 16 | Journal Issue: | 6 | Start page/Pages: | 776-787 | Source: | Gene Therapy | Abstract: | Genetic immunization strategies have largely focused on the use of plasmid DNA with a gene gun. However, there remains a clear need to further improve the efficiency, safety, and cost of potential DNA vaccines. The gold particle-coated DNA format delivered through a gene gun is expensive, time and process consuming, and raises aseptic safety concerns. This study aims to determine whether a low-pressured gene gun can deliver noncarrier naked DNA vaccine without any particle coating, and generate similarly strong antigen-specific immunologic responses and potent antitumor effects compared with gold particle-coated DNA vaccine. Our results show that mice vaccinated with noncarrier naked chimeric CRT/E7 DNA lead to dramatic increases in the numbers of E7-specific CD8+ T-cell precursors and markedly raised titers of E7-specific antibodies. Furthermore, noncarrier naked CRT/E7 DNA vaccine generated potent antitumor effects against subcutaneous E7-expressing tumors and pre-established E7-expressing metastatic pulmonary tumors. In addition, mice immunized with noncarrier naked CRT/E7 DNA vaccine had significantly less burning effects on the skin compared with those vaccinated with gold particle-coated CRT/E7 DNA vaccine. We conclude that noncarrier naked CRT/E7 DNA vaccine delivered with a low-pressured gene gun can generate similarly potent immunologic responses and effective antitumor effects has fewer side effects, and is more convenient than conventional gold particle-coated DNA vaccine. |
URI: | 2-s2.0-67349215725 https://scholars.lib.ntu.edu.tw/handle/123456789/458631 |
ISSN: | 0969-7128 | DOI: | 10.1038/gt.2009.31 | SDG/Keyword: | calreticulin; calreticulin protein E7 DNA vaccine; DNA vaccine; gold nanoparticle; naked DNA; protein E7; unclassified drug; animal cell; animal experiment; animal model; animal tissue; antigen specificity; antineoplastic activity; article; cancer gene therapy; cancer immunotherapy; cancer prevention; CD8+ T lymphocyte; chimera; controlled study; DNA immunization; drug coating; drug dosage form comparison; drug megadose; female; gene gun; immune response; injection site burning; local skin reaction; lung metastasis; mouse; neoplasm; nonhuman; priority journal; prolymphocyte; skin defect; subcutaneous tissue tumor; vaccination reaction; Animals; Antigens, CD11c; Biolistics; Buffers; Burns, Chemical; Cancer Vaccines; CD8-Positive T-Lymphocytes; Cell Line, Tumor; Cells, Cultured; Dendritic Cells; Dermis; Dose-Response Relationship, Immunologic; Drug Carriers; Enzyme-Linked Immunosorbent Assay; Epitopes, T-Lymphocyte; Female; Flow Cytometry; Gold; Immunotherapy; Injections, Intradermal; Luciferases; Mice; Mice, Inbred C57BL; Microscopy, Fluorescence; Neoplasms; Neoplasms, Experimental; Papillomavirus E7 Proteins; Pressure; Recombinant Fusion Proteins; Vaccines, DNA; Mus [SDGs]SDG3 |
Appears in Collections: | 醫學系 |
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