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  1. NTU Scholars
  2. 醫學院
  3. 醫學系
Please use this identifier to cite or link to this item: https://scholars.lib.ntu.edu.tw/handle/123456789/458681
Title: Cancer immunotherapy using sindbis virus replicon particles encoding a VP22-antigen fusion
Authors: WEN-FANG CHENG 
Hung C.-F.
Hsu K.-F.
Chai C.-Y.
He L.
Polo J.M.
Slater L.A.
Ling M.
Wu T.-C.
Issue Date: 2002
Journal Volume: 13
Journal Issue: 4
Start page/Pages: 553-568
Source: Human Gene Therapy
Abstract: 
Alphavirus vectors have emerged as a strategy for the development of cancer vaccines and gene therapy applications. The availability of a new packaging cell line (PCL), which is capable of generating alphavirus replicon particles without contamination from replication-competent virus, has advanced the field of vaccine development. This replication-defective vaccine vector has potential advantages over naked nucleic acid vaccines, such as increased efficiency of gene delivery and large-scale production. We have developed a new strategy to enhance nucleic acid vaccine potency by linking VP22, a herpes simplex virus type 1 (HSV-1) tegument protein, to a model antigen. This strategy facilitated the spread of linked E7 antigen to neighboring cells. In this study, we created a recombinant Sindbis virus (SIN)-based replicon particle encoding VP22 linked to a model tumor antigen, human papillomavirus type 16 (HPV-16) E7, using a stable SIN PCL. The linkage of VP22 to E7 in these SIN replicon particles resulted in a significant increase in the number of E7-specific CD8+ T cell precursors and a strong antitumor effect against E7-expressing tumors in vaccinated C57BL/6 mice relative to wild-type E7 SIN replicon particles. Furthermore, a head-to-head comparison of VP22-E7-containing naked DNA, naked RNA replicons, or RNA replicon particle vaccines indicated that SINrep5-VP22/E7 replicon particles generated the most potent therapeutic antitumor effect. Our results indicated that the VP22 strategy used in the context of SIN replicon particles may facilitate the generation of a highly effective vaccine for widespread immunization.
URI: 2-s2.0-0036204348
https://scholars.lib.ntu.edu.tw/handle/123456789/458681
ISSN: 1043-0342
DOI: 10.1089/10430340252809847
SDG/Keyword: cancer vaccine; CD8 antigen; naked DNA; RNA; tumor antigen; virus vector; Alpha virus; animal cell; antineoplastic activity; article; cancer immunotherapy; cell line; controlled study; DNA replication; gene targeting; Herpes simplex virus 1; immunization; mouse strain; nonhuman; packaging; replicon; RNA replication; Sindbis virus; stem cell; T lymphocyte; vaccination; viral contamination; virus recombinant; virus replication; Wart virus; Animals; Cancer Vaccines; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Female; Gene Transfer Techniques; Immunotherapy; Mice; Mice, Inbred C57BL; Neoplasms; Oncogene Proteins, Viral; Replicon; Sindbis Virus; Vaccines, DNA; Viral Proteins; Alphavirus; Animalia; Human herpesvirus 1; Human papillomavirus; Human papillomavirus type 16; Human papillomavirus types; Papillomavirus; Simplexvirus; Sindbis virus
[SDGs]SDG3
Appears in Collections:醫學系

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臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

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