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  1. NTU Scholars
  2. 醫學院
  3. 醫學系
Please use this identifier to cite or link to this item: https://scholars.lib.ntu.edu.tw/handle/123456789/458803
Title: Zinc oxide nanoparticles-induced intercellular adhesion molecule 1 expression requires Rac1/Cdc42, mixed lineage kinase 3, and c-Jun N-terminal kinase activation in endothelial cells
Authors: Li C
Liao P
MING-KWANG SHYU 
Liu C
Kao C
Huang S
Cheng Y
Kang J.-J.
Issue Date: 2012
Journal Volume: 126
Journal Issue: 1
Start page/Pages: 162-172
Source: Toxicological Sciences
Abstract: 
The explosive development of nanotechnology has caused an increase in unintended biohazards in humans and in the ecosystem. Similar to particulate matter, nanoparticles (NPs) are strongly correlated with the increase in incidences of cardiovascular diseases, yet the mechanisms behind this correlation remain unclear. Within the testing concentrations of 0.1-10 μg/ml, which did not cause a marked drop in cell viability, zinc oxide NPs (ZnO-NPs) induced intercellular adhesion molecule-1 (ICAM-1) messenger RNA, and protein expression in both concentration- and time-dependent manner in treated human umbilical vein endothelial cells (HUVECs). ZnO-NPs treatment cause the activation of Ras-related C3 botulinum toxin substrate 1 (Rac1)/cell division control protein 42 homolog (Cdc42) and protein accumulation of mixed lineage kinase 3 (MLK3), followed by c-Jun N-terminal kinase (JNK) and transcription factor c-Jun activation. Induction of ICAM-1 and phosphorylation of JNK and c-Jun could be inhibited by either JNK inhibitor SP600125 or Rac guanosine triphosphatase inhibitor NSC23766 pretreatment. In addition, pretreatment with NSC23766 significantly reduced MLK3 accumulation, suggesting the involvement of Rac1/Cdc42-MLK3-JNK-c-Jun signaling in the regulation of ZnO-NPs-induced ICAM-1 expression, whereas these signaling factors were not activated in zinc oxide microparticles (ZnO-MPs)-treated HUVECs. The increase of ICAM-1 expression on ZnO-NPs-treated HUVECs enables leukocytes to adhere and has been identified as an indicator of vascular inflammation. Our data are essential for safety evaluation of the clinical usage of ZnO-NPs in daily supplements, cosmetics, and biomedicines. ? The Author 2011. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved.
URI: https://scholars.lib.ntu.edu.tw/handle/123456789/458803
ISSN: 1096-6080
DOI: 10.1093/toxsci/kfr331
SDG/Keyword: 4 amino 6 [2 [[4 (diethylamino) 1 methylbutyl]amino] 6 methyl 4 pyrimidinyl] 2 methylquinoline; anthra[1,9 cd]pyrazol 6(2h) one; intercellular adhesion molecule 1; messenger RNA; mixed lineage kinase 3; nanoparticle; protein Cdc42; Rac1 protein; stress activated protein kinase; unclassified drug; zinc oxide; zinc oxide nanoparticle; animal experiment; animal tissue; article; cell membrane permeability; concentration response; controlled study; enzyme inhibition; enzyme phosphorylation; gene expression regulation; gene induction; human; human cell; in vivo study; leukemia cell; leukocyte adherence; monocyte; mouse; nonhuman; particle size; protein content; protein expression; protein induction; protein localization; signal transduction; transcription initiation; umbilical vein endothelial cell
[SDGs]SDG3
Appears in Collections:醫學系

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臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

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